苏太猪SLA-DQB和SLA-DRB基因第2外显子多态性及其与繁殖性能的关联分析  

Polymorphism of Exon 2 of SLA-DQB and SLA-DRB Genes and Its Relationship for Productive Performance in Sutai Pigs

作　　者：吴圣龙[1] 鞠慧萍[1] 孙鹏翔[1] 黄军[1] 包文斌[1] 华金弟 黄雪根 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省苏州市苏太猪育种中心,苏州215128

出　　处：《农业生物技术学报》2007年第4期606-611,共6页Journal of Agricultural Biotechnology

基　　金：江苏省高技术研究发展计划项目(No.BG2006302);扬州大学自然科学基金项目(No.NK0513119)资助

摘　　要：采用PCR-RFLP技术对苏太猪SLA-DQB及SLA-DRB基因第2外显子PCR产物进行分析。结果表明,苏太猪SLA-DQB基因经RsaⅠ酶切后共产生3种等位基因,5种基因型;SLA-DRB基因经RsaⅠ酶切后共产生3种等位基因,4种基因型。χ2适合性检验表明,苏太猪SLA-DQB及SLA-DRB基因外显子2的RsaⅠ酶切位点均达到了Hardy-Weinberg平衡状态。将SLA-DQB和SLA-DRB基因的基因型进行组合,共产生12种组合基因型,BBDF的第3胎繁殖性能总产仔数、产活仔数、初生窝重和断奶仔猪数显著高于组合基因型AADD、AADF、ABDD和CCDF(P<0.05)。从繁殖性能看,BBDF在群体中为最优秀的组合基因型。The exon 2 of SLA （swine leukocyte antigen）-DQB and SLA-DRB genes in Sutai pigs was analyzed by PCR-RFLP method, and the results showed that the exon 2 of SLA-DQB gene digested by Rsa Ⅰ could be divided into three kinds of alleles and five kinds of genotypes, the exon 2 of SLA-DRB gene digested by Rsa Ⅰ could be divided into three kinds of alleles and four kinds of genotypes. The results of Chi Square test indicated that the frequencies of patterns types of SLA-DQB and SLA-DRB genes digested by Rsa Ⅰ were fit with Hardy-Weinberg equilibrium in Tibet pig populations. There were twelve combined patterns of SLA-DQB and SLA-DRB genes in Sutai pigs, the correlation between these combined patterns with the productive performances of Sutai pigs was analyzed. The results showed that productive performances （the total litter, number of viable pigs, litter weight at birth and number of ablactation pigs in the third litter ） of Sutai pigs with BBDF combined pattern were significantly higher than those with AADD, AADF, ABDD and CCDF combined pattern （P 〈0.05）, respectively. As the productive performances of Sutai pigs, the combined pattern of BBDF was the most favorable.

关 键 词：苏太猪 SLA-DQB基因 SLA-DRB基因 合并基因型 繁殖性能 

分 类 号：S188[农业科学—农业基础科学]