草莓乙烯受体FaEtr1和FaErs1基因的克隆及其反义表达载体的构建  

Cloning of Ethylene Receptor Genes of FaEtr1 and FaErs1 in Strawberry and Construction of Their Plant Antisense Expression Vectors

作　　者：朱海生[1] 温庆放[2] 林义章[1] 张志忠[1] 潘东明[1] 

机构地区：[1]福建农林大学园艺学院,福州350002 [2]福建省农业科学院蔬菜研究中心

出　　处：《农业生物技术学报》2007年第4期684-688,共5页Journal of Agricultural Biotechnology

基　　金：福建省财政专项-福建省农业科学院科技创新团队建设基金(No.STIF-Y06)资助

摘　　要：在已报道的FaEtr1和FaErs1基因序列的基础上,设计特异引物,分别克隆草莓(Fragaria ananassa)乙烯受体FaEtr1基因580bp部分特异序列和FaErs1基因445bp部分特异序列,将上述2个片段分别反向插入植物表达载体pBI121的CaMV35S启动子和Nos终止子之间,构建了反义表达载体pBI121Etr1和pBI121Ers1。将带有完整启动子和终止子的FaEtr1和FaErs1基因引入pCAMBIA1301中,最终获得FaEtr1和FaErs1的双价植物表达载体pFRS。将这3个重组表达质粒pBI121Etr1、pBI121Ers1和pFRS导入根癌农杆菌(Agrobactrium tumefaciens)EHA105中,PCR及限制性内切酶酶切确定质粒已被导入。By using the specific primers designed from the FaEtrl eDNA and the FaErsl cDNA, 580 bp fragment of FaEtrl and 445 bp fragment of FaErsl were cloned. The FaEtrl and FaErsl cDNA were inserted into reverse orientation between the CaMV 35S promoter and Nos terminator of the expression vector pBI121, respectively, and two antisense expression vectors pBI121Etrl and pBI121Ersl were obtained. FaEtrl and FaErsl antisense genes with 35S promoter and Nos terminator were inserted into expression vector pCAMBIA1301 together to generate double genes plant expression vector pFRS. Vectors of pBI121Etrl, pBI121Ersl and pFRS were transformed into Agrobacttium tunefaciens EHA 105. Correct construction and transformation were confirmed by PCR and restriction enzymes analysis.

关 键 词：乙烯受体 草莓 反义表达载体 

分 类 号：S188[农业科学—农业基础科学]