外伤后细菌性致死性肉芽肿复发后病原体药敏试验及形成生物膜的能力  

The drug susceptibility and biofilm formation of the pathogen cultured from a patient with recurrent fatal bacterial granuloma after trauma

作　　者：齐显龙[1] 孙东杰[1] 高剑[1] 徐修礼[2] 林琨[1] 李春英[1] 高天文[1] 

机构地区：[1]第四军医大学西京医院全军皮肤病研究所,西安710032 [2]第四军医大学西京医院检验科,西安710032

出　　处：《中国麻风皮肤病杂志》2007年第9期756-758,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:检测外伤后细菌性致死性肉芽肿复发后分离菌株对常用抗生素的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)及其形成生物膜(biofilm,BF)的能力。方法:肉汤稀释法测定两次复发后分离培养的痤疮丙酸杆菌标准株(NCTC737)的MIC及MBC,XTT法测定BF形成的能力。结果:第二次复发后病原体林可霉素的MIC升高两个浓度级;该患者第二次病原菌形成BF的能力强于初次复发的病原体。结论:长期抗生素治疗可能导致FBGT病原菌对某些抗生素MIC升高,同时增加其形成BF的能力。Objective： To determine the MIC and MBC for commonly used antibiotics and the ability in biofilm formation of two isolates from a patient with recurrent fatal bacterial granuloma after trauma （FBGT）. Methods： The MIC and MBC levels were measured with broth dilution method according to the NCCLS, and the ability of biofilm formation was measured with XTT method. Results： The MIC level of lincomycin elevated 2 density grades and the ability of biofilm formation was stronger for the isolate in the second recurrence, compared with the isolate in the first recurrence. Conclusion： Long term use of antibiotic may elevate the MIC level to the pathogen of FBGT and increases the ability in biofilm formation.

关 键 词：痤疮丙酸杆菌 生物膜 外伤后细菌性致死性肉芽肿 最小抑菌浓度 

分 类 号：R446.5[医药卫生—诊断学]