APP17肽对紫外线照射的皮肤成纤维细胞MMP-1和TIMP-1 mRNA表达的影响  

The effects of APP17-mer peptide on the expression of MMP-1 and TIMP-1 in cultured human skin fibroblasts irradiated with ultraviolet

作　　者：陈慧[1] 朱威[1] 连石[1] 王蓉[2] 张景艳[2] 姬志娟[2] 

机构地区：[1]首都医科大学宣武医院皮肤性病科,北京100053 [2]首都医科大学宣武医院教育部神经变性病重点实验室,北京100053

出　　处：《中国麻风皮肤病杂志》2007年第9期759-762,共4页China Journal of Leprosy and Skin Diseases

摘　　要：目的:探讨β-淀粉样前体蛋白17肽(APP17肽)对紫外线照射后人皮肤成纤维细胞增殖及MMP-1和TIMP-1mRNA表达的影响。方法:在培养的人皮肤成纤维细胞中加入不同浓度的APP17肽,并用UV照射培养的人皮肤成纤维细胞。MTT法检测细胞活性,应用RT-PCR方法检测不同剂量APP17肽及UV照射后细胞中MMP-1mRNA和TIMP-1mRNA的表达情况。结果:APP17肽可增加非照射及UV照射后成纤维细胞的活性(P<0.05)。RT-PCR结果显示,6J/cm2UVA+36mJ/cm2UVB照射后MMP-1mRNA的表达增加1.78倍(P<0.01),TIMP-1mRNA的表达增加1.13倍(P<0.05)。APP17肽可在一定水平抑制MMP-1mRNA的表达,并诱导TIMP-1mRNA的表达(P<0.05)。结论:APP17肽可抑制体外培养的成纤维细胞的胶原降解,并可抑制UV诱导的胶原降解。Objective： To determine the effects of β - amyloid precursor protein 17 - met peptide （APP17） on the proliferation and MMP- 1 mRNA and TIMP- 1 mRNA expressions in cultured fibroblasts after ultraviolet irradiation. Methods： Human skin fibroblasts were cultured in a series of concentrations of APP 17 - mer peptide （0,20, 40,80 μmol/L, respectively） and irradiated by a single exposure to ultraviolet. The activities of fibroblasts were detected by the assay of MTr. The expressions of MMP- 1 and TIMP- 1 were analyzed by real - time quantitative RT - PCR in APP17 treated and 9 J/cm^2 UVA ＋ 54 mJ/cm^2 UVB irradiated fibroblasts. Results： After treatment with APP17, the activities of fibroblasts increased in both unirradiated and UV - irradiated skin （ P 〈 0.05）. A single exposure of fibroblasts to 6 J/cm^2 UVA ＋ 36 mJ/cm^2 UVB irradiation resulted in a 1.78 - fold up - regulation of MMP- 1 mRNA and a 1.13 - fold up - regulation of TIMP - 1 mRNA compared with unirradiated control （ P 〈 0.01, P 〈 0.05 respectively）. 40 μmol/L and 80 μmol/L APP17 decreased the expression of MMP - 1 mRNA （ P 〈0.05, P 〈 0.01 respectively） and increased the expression of TIMP - 1 mRNA （P 〈 0.05）. Conclusion： APP17 can inhibit collagen degradation in unirradiated and irradiated fibroblasts with ultraviolet.

关 键 词：APP17肽 成纤维细胞 实时定量RT-PCR 

分 类 号：R751[医药卫生—皮肤病学与性病学]