11β羟化类固醇脱氢酶1与成骨细胞分化之间相互关系的研究  

The research of 11β-hydroxysteroid dehydrogenase 1 in the mechanism of osteoblastic differentiation

作　　者：孙岩[1] 程鹏[1] 张爱森[1] 俞静[1] 谢宇[1] 朱亭[1] 刘娟[1] 刘云[1] 丁国宪[1] 

机构地区：[1]南京医科大学第一附属医院老年医学科,210008

出　　处：《中国骨质疏松杂志》2007年第3期163-168,共6页Chinese Journal of Osteoporosis

摘　　要：目的通过体外地塞米松刺激原代大鼠成骨细胞分化,观察11β羟化类固醇脱氢酶1(11β-HSD1)在成骨细胞分化中的变化以及相关成骨基因的表达情况,探讨11β-HSD1与成骨细胞分化之间关系以及可能的作用机制。方法采用酶消化法获得大鼠成骨细胞,建立地塞米松(Dex)药物刺激模型(Dex组1×10-8mol/L和正常对照组),分别于培养0、2、4、6、8、10d抽提RNA行RT-PCR和蛋白行Western Blot,检测成骨细胞相关基因和11β-HSD1的表达情况。结果11β-HSD1在正常成骨细胞中随着培养表达逐渐升高,在Dex刺激组中成骨细胞分化指标ALP、COLⅠ较对照组明显升高,伴有11β-HSD1 mRNA和蛋白水平的下调。结论在成骨分化过程中,11β-HSD1表达下降,与分化呈逆相关。11β-HSD1可能通过自分泌的方式参与调节成骨细胞的分化。Objective To investigate the relationship between 11β-hydroxysteroid dehydrogenase 1 and osteoblastic differentiation. Methods The primary osteoblasts were obtained by sequential enzymatic digestion of fetal rat calvaria and the models of drug-stimulated by Dex （1 × 10^-8 mol/L） and the control was built. We detected the expression of the osteoblast related genes and 11β-HSD1 mRNA and protein by RT-PCR and Western blot. Results 11β-HSD1 was up-regulated in the course of primary culture. In the Dex-stimulated models, ALP and COL I were obviously up-regulated compared to the unstimulated group. The mRNA and protein levels of 11β-HSD1 were down-regulated in the Dex-stimulated models. Conclusions The expression of 11β-HSD1 is closely related to osteoblastic differentiation. During the differentiation process, the expression of 11β-HSD1 decreased. In osteoblasts, there may be a negative relation between 11β-HSD1 expression and cell differentiation. 　

关 键 词：11β羟化类固醇脱氢酶1 成骨细胞 地塞米松 细胞分化 

分 类 号：R336[医药卫生—人体生理学]