联合运用重组人生长激素和雌激素对去势大鼠骨密度及生物力学的影响  

The effects of rh-GH and estrogen on mandible and femur in ovariectomized rats bone dense and maximum load

作　　者：胡亚军[1] 郭三萍[1] 李晓红[1] 唐成芳[2] 肖卓[1] 

机构地区：[1]西安交通大学口腔医院修复科,西安710004 [2]西安医学院

出　　处：《中国骨质疏松杂志》2007年第2期91-95,共5页Chinese Journal of Osteoporosis

基　　金：陕西省科学技术研究发展计划项目(2003k10-G78)

摘　　要：目的研究重组人生长激素和雌激素联合用药对去势大鼠股骨、下颌骨骨密度及生物力学的影响。方法选4月龄SD雌性大鼠,随机分为5组:假手术组(sham-OVX)、骨质疏松组(OVX)、雌激素组(E2)、重组人生长激素组(rh-GH)、联合用药组(E2+rh-GH),于去势后8w,各组分别腹腔给予相应的药物,4w末及8w末分两批处死各组大鼠,取股骨和下颌骨新鲜标本,进行骨密度(BMD)与最大载荷的实验研究。结果用药4w末及8w末,OVX组下颌骨和股骨BMD均显著低于其他4组。E2+rh-GH组下颌骨BMD较E2组和rh-GH组高,但是差异均无统计学意义(P>0.05)。用药4w末及8w末,OVX组下颌骨和股骨最大载荷均显著低于sham-OVX组和用药组。E2+rh-GH组下颌骨及股骨最大载荷比E2组、rh-GH组高,用药4w末联合用药组与单一用药组最大载荷的差异有统计学意义;而用药后8w末差异均无统计学意义。结论雌激素、重组人生长激素及两者联合用药都能有效的抑制骨质疏松大鼠颌骨及股骨骨量的丢失,提高骨强度。联合用药与单一用药对下颌骨骨密度的影响没有明显的差异,可能与测量的方法和测量部位结构有关。联合用药对股骨骨密度、最大载荷及颌骨最大载荷的作用与用药时间有关,短期内效果优于单独用药。Objective To explore combined effect of rh-GH and estrogen on mandible and femur＇s BMDs and maximum load in ovariectomized rats. Methods Sixty five 4-month-old SD rats were randomly divided into 5 groups ： sham, pop, E2, rh-GH, E2 ＋ rh-GH. 8 weeks after ovariectomy or sham-ovariectomy, rats in Estrogen group were injected in estrogen 10μg/kg every three days; recombinant human growth hormone group were injected in recombinant human growth horuaone 0.15 IU/kg every day. Drug combination group were injected in estrogen 10 μg/kg ever, three days and recombinant human growth hormone 0.15 IU/kg every day. The rest of the groups were injected in the same amount of sodium chloride. Treated for 4 weeks, half of the rats were sacrificed, and the rest were put to death 4 weeks later. The left femur and left mandible were collected for determination of BMD and maximum load. Results The ovariectomized rats were obviously heavier than sham-ovariectomized rats. After having been treated for 4 weeks, compared with POP group, treated groups and sham group were significantly higher in BMD and maximum load（ P 〈 0.05） . Compared with other two treated groups, as for femur＇s BMD, E2＋ rh-GH group was significantly higher than that of rh-GH group and E2 group after treated for 4 weeks; but only significantly higher than E： group after treated 8 weeks. There was no statistical significance in mandible＇s BMD among three treated groups. After 4 weeks＇ therapy, maximum loads of mandible and femur of E2 ＋ rh-GH group were significantly higher than other two treated groups. After 8 weeks＇ therapy, there was no statistical significance among three treated groups in maximum load. Conclusions Femur and mandible＇s bone mass and bone strength in ovariectomized rats have significantly decreased, After being treated, bone mass and bone strength can obviously increase. Combined effect of estrogen and recombinant human growth hormone on mandible BMD has no obvious difference from the single effect. That may be led to by

关 键 词：重组人生长激素 雌激素 绝经后骨质疏松 最大载荷 骨密度 三点弯曲 双能X线骨密度测定 

分 类 号：R644[医药卫生—外科学]