BEMF对去卵巢骨质疏松大鼠骨组织bFGF表达的影响及意义  

Impact of bionics pulsed electromagnetic fields on the expression of bFGF in the bone tissue of ovariectomy osteoporosis rats

作　　者：谢肇[1] 李起鸿[1] 许建中[1] 孟萍[1] 

机构地区：[1]第三军医大学附属西南医院骨科,重庆400038

出　　处：《中国骨质疏松杂志》2007年第2期96-100,共5页Chinese Journal of Osteoporosis

摘　　要：目的观察BEMF对去卵巢骨质疏松大鼠骨组织bFGF蛋白表达的影响,探讨BEMF治疗骨质疏松的机理。方法6月龄雌性未孕Wistar大鼠40只,按体质量随机分为模型组(OVX)、假手术组(Sham)、仿生脉冲电磁场治疗组(BEMF+OVX,EM)、雌激素治疗组(Estrogen+OVX,E)。OVX、EM、E组行双侧卵巢切除术,Sham组行假手术。术后第9W开始治疗:E组行苯甲酸雌二醇肌肉注射,0.5mg/kg,1次/2W。EM组大鼠暴露于仿生脉冲电磁场治疗,1h/1次/d,OVX、Sham组不予以任何处理,作为对照组。治疗10W后处死各组实验动物,测量腰椎骨密度、椎体的最大载荷、扫描电镜观察椎体骨结构的变化。采用免疫组化检测椎体骨组织中bFGF蛋白表达情况并以图像分析进行组间比较。结果治疗10W后EM组大鼠骨密度、椎体的最大载荷较OVX组显著性增高(P<0.05),骨结构明显改善。bFGF的表达主要位于成骨细胞、骨细胞,EM组椎体骨组织中bFGF表达显著性高于OVX组(P<0.01)。结论提高骨组织中bFGF的表达是BEMF治疗骨质疏松的重要机理之一。Objective Observing the impact of bionics pulsed electromagnetic fields on of bFGF protein expression in the bone tissue of Ovariectomy Osteoporosis Rats and to explore the mechanism of the therapeuetic effect of bionics electromagnetic fields in postmanupausal osteoporosis. Methods Forty 6-month old female Wistar rats were randomly divided into four different groups according to body mass： ovariectomy group（OVX）, sham operation group （Sham）, E group （Estrogen ＋ OVX） and EM group（ BEMF ＋ OVX）. All rats were subjected to bilateral ovariectomy except sham operation group. After 8 weeks operation, E group rats were given estrogen 0.5 mg/kg, 1/ 2 W. EM group rats were exposed to bionics electromagnetic fields, 1h/d. OVX and Sham group rats were given nothing. All the treatments were bing kept for10 weeks. After the treatments finished, meauring the markers as follow： the bone mineral density （BMD）（lumbar vertebrae）, the maximum load of lumbar vertebrae, bone morphology （lumbar vertebrae） were observed by scan electric microscope. The expression of bFGF protein with immunochemistry. Results Compared with OVX group, BMD and the maximum load significantly increased at the lumbar vertebrae in EM group（ P 〈 0.05）. Scan electric microscope observation showed trabecular volume and trabecular connections increased in EM group than in OVX. Imaging analyses showed that BEMF stimulation exposure significantly elevated the expression of bFGF protein than that in OVX group. Conclusions BEMF are useful in the treatment of postmanupausal osteoporosis resulting from ovariectomy. What the BEMF stimulation exposure （tone, facilitating bFGF protein expression in bone tissue may be one of mechanisms of therapeutic efficacy of BEMF on postmanupausal osteoporosis.

关 键 词：仿生脉冲电磁场 绝经后骨质疏松 碱性成纤维细胞因子 

分 类 号：R783.302[医药卫生—口腔医学]