一种光学显微自动聚焦系统设计与实现  

Design and implementation of optical auto-focus microscopy

作　　者：易秋实[1] 张红民[1] 吴萍[1] 吕晓华[1] 骆清铭[1] 曾绍群[1] 

机构地区：[1]华中科技大学生物医学光子学教育部重点实验室-武汉光电国家实验室,武汉430074

出　　处：《计算机工程与应用》2007年第27期119-120,215,共3页Computer Engineering and Applications

基　　金：国家自然科学基金(the National Natural Science Foundation of China under Grant No.60410131;No.90508003)

摘　　要：针对普通CCD拍摄的低对比度生物样品图像,设计并实现了一种以PC机为控制核心的光学显微镜自动聚焦系统。系统采用弹性联轴器与显微镜调焦轴直接耦合的方式,结合细分驱动,有效地消除了传动机构的回程差,提高了聚焦控制精度。在对几种常用图像清晰度评价算子进行分析的基础上,证实了图像自相关算子是评价低对比度生物样品图像清晰度的有效算法。实验结果表明,该系统自动聚焦的准确度和实时性基本上满足生物学研究的需要。A novel optical auto-focus microscopy controlled by PC is presented,especially for the low-contrast images from con- ventional CCD camera.Firstly,by directly matching flexible shaft coupling and focusing axis of microscope,integrating subdivision mechanism of micro-step driver,the mechanical tolerance of gear was diminished while the accuracy of focus was improved effectively.Secondly,it has been proved that the auto-correlation operator is one effective approach to evaluate low-contrast image specially based on the performance comparison of some image analysis operators.Biology experiments have shown that the pro- posed scheme meets the requirement of auto-focusing both on accuracy and speed.

关 键 词：自动聚焦 显微镜 步进电机 自相关算子 

分 类 号：TP302.1[自动化与计算机技术—计算机系统结构] TH742[自动化与计算机技术—计算机科学与技术]