华夏小葱制剂对脂肪肝大鼠脂质代谢和组织形态学的影响  

Effects of a preparation of Chinese Allium fistulosum L on the morphology and metabolism of lipids in fatty liver rats

作　　者：张介眉[1] 郝建军[1] 时昭红[1] 常青[2] 邴飞虹[2] 

机构地区：[1]武汉市中西医结合医院,湖北省武汉市430022 [2]湖北中医学院中药研究所,湖北省武汉市430061

出　　处：《世界华人消化杂志》2007年第22期2447-2452,共6页World Chinese Journal of Digestology

基　　金：湖北省卫生科研基金项目;No.2006-63~~

摘　　要：目的:观察华夏小葱制剂对脂肪肝大鼠防治作用及其对组织形态学影响.方法:60只SD大鼠随机分为空白对照组(n= 10),模型对照组(n=10),华夏小葱制剂低剂量组(n=10),中剂量组(n=10),高剂量组(n =10)以及东宝肝泰片组(n=10)。空白对照组用普通饲料及普通水喂养;其他组用高脂饮食及酒精水喂养,并结合每周一次行后肢sc小剂量四氯化碳色拉油溶液的方法建立大鼠脂肪肝模型.用光镜及电镜观察肝脏的形态学改变情况,采用全自动生化分析仪测定总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇fLDL-C),高密度脂蛋白胆固醇(HDL-C),ALT(丙氨酸氨基转移酶),AST(天门冬酸氨基转移酶),TP(总蛋白),ALB(清蛋白)、GLO(球蛋白)及A/G(清蛋白/球蛋白)的含量.结果:光镜和电镜观察均见模型对照组有严重脂肪变性,脂滴增多;华夏小葱低、中、高剂量组及东宝肝泰片组脂肪变性程度有所减轻,脂滴不同程度的有所减少.和空白对照组的TC,TG,HDL-C,LDL-C,ALT,AST,TP,ALB相比,模型对照组大鼠血浆TC,TG,LDL-C,ALY,AST值均显著升高(P<0.01),HDL-C,TP,ALB值均显著降低(P<0.01);和模型对照组的TC,TG,HDL-C,LDL-C,ALT,AST TP,ALB相比,华夏小葱低,中,高剂量组及东宝肝泰片组TC,TG,LDL-C值均显著降低(TC:低,中,高剂量组P<0.05,东方肝泰片组P<0.01;TG:低,中剂量组和东方肝泰片平P<0.05,高剂量细P<0.01:LDL-C:均P<0.05),HDL-C值与模型对照组相比显著升高(均P<0.05),ALY,AST值与模型对照组相比无统计学差异,华夏小葱高剂量组与模型对照组相比ALB值显著升高(P<0.05).结论:华夏小葱制剂能改善脂肪肝大鼠体内的脂质代谢,对脂肪肝有一定的防治作用.AIM： To study the preventive and therapeutic effects of a preparation of Chinese Allium fistulosum L （PCAL） on fatty liver rats.METHODS： Sixty sprague-dawley rats were randomly divided into six groups; a blank con- trol group （n = 10）, model control group （n = 10）, low, medium and high-dose PCAL （PCAL low, PCAL medium and PCAL high, respectively, n = 10 in each group）, and a Dongbaogantai group （n = 10）. The blank control group was given water and food. Other groups were fed with a high-fat diet, given water with 15% ethanol, and treated with a small dose of carbon tetrachloride （CC14） hypodermically once a week to establish the fatty liver model. All animals were sacrificed after 8 wk. Plasma total cholesterol （TC）, triglyceride （TG）, high-density lipoprotein-cholesterol （HDL-C）, low-density lipoprotein-cholesterol （LDL-C）, alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, total protein （TP）, albumin （ALB）, globulin （GLO）, and A/G （albumin/globulin） were determined by an automatic biochemistry analyzer. Hematoxylin-eosin （HE） staining and electron microscopy were used to detect changes in the hepatic pathology of the fatty liver rats. RESULTS： From HE staining and electron microscopy, we ascertained the phenomena of severe fatty degeneration and the multiplication of lipid droplets in the model control group. The extent of fatty degeneration and multiplication of lipid droplets in the PCAL low, middle and high groups, and the Dongbaogantai group was relatively mitigated. Compared with the blank control group, the level of TC, TG, LDL-C, ALT and AST in the model control group increased significantly （all P 〈 0.01）; the level of HDL-C, TP and ALB in the model control group decreased significantly （all P 〈 0.01）; the level of TC, TG and LDL-C in the PCAL low, middle and high groups and the Dongbaogantai group decreased significantly （TC, PCAL low, middle and high group, P 〈 0.05; Dongbaogantai group, P 〈 0.01

关 键 词：华夏小葱制剂 脂肪肝 形态学 脂质代谢 全自动生化分析仪 透视电镜 

分 类 号：R285.5[医药卫生—中药学]