湛江等鞭金藻对抗生素的反应及无菌化培养  

Effects of antibiotics on the growth of Isochrysis zhangjiangensis and axenic culture

作　　者：黄振华[1] 刘晓娟[1] 胡章喜[1] 段舜山[1] 

机构地区：[1]暨南大学水生生物研究所,广州510632

出　　处：《生态科学》2007年第2期120-121,125,共3页Ecological Science

基　　金：国家自然科学基金(30370231);广东省科技计划重点引导项目(2005B33201001);珠海市科技计划重大项目(PB20041018)

摘　　要：研究了5种抗生素在不同浓度下对湛江等鞭金藻(Isochrysis zhangjiangensis)生长的影响,采用混合添加抗生素法获得无菌藻株。结果表明,(1)不同抗生素对湛江等鞭金藻生长的影响存在差异,湛江等鞭金藻对氯霉素最敏感,浓度为50μg·mL-1时抑制率为27.02%,浓度>50μg·mL-1时抑制率达80%,湛江等鞭金藻对青霉素、链霉素、卡那霉素和庆大霉素不敏感,后3种抗生素在低浓度时对藻细胞生长有促进作用;(2)除50μg·mL-1青霉素处理外,青霉素、链霉素、卡那霉素和庆大霉素其它浓度处理均有明显的抑菌作用;(3)采用500μg·mL-1链霉素,1000μg·mL-1卡那霉素和50μg·mL-1庆大霉素的4个组合混合添加抗生素,均获得了无菌藻株,且对生长有促进作用。Effects of 5 kinds of antibiotics （chloramphenicol, ampicillin, streptomycin, kanamycin, gentamycin） at different concentrations on the growth ofIsochrysis zhangjiangensis were studied, the axenic culture was obtained by mixed antibiotics. The results showed that： （1） There were differences among different antibiotics, chloramphenicol was the most susceptible antibiotic, the inhibition rate was 27.02% at the concentration of 50 μg.mL^-1, more than 50 μg.mL^-1 the inhibition rate was 80%. Lzhangjiangensis was not susceptible to ampicillin, streptomycin, kanamycin and gentamycin, there was stimulation at Re lower concentration of the last three antibiotics. （2） Except for ampicillin at the concentration of 50 μg.mL^-1, there were bacteriostasis effects of the other treatments. （3） Mixed antibiotics （500 μg.mL^-1 streptomycin, 1000 μg.mL^-1 kanamycin, and 50 μg.mL^-1 gentamycin） were added to the culture medium by 4 combinations, the axenic culture was obtained, and there were stimulation on the growth of Lzhangjiangensis.

关 键 词：湛江等鞭金藻 抗生素 无菌 

分 类 号：Q949.262[生物学—植物学]