迎头色谱法测定人参皂苷Rg_1和Re的吸附量  

Determination of adsorption of ginsenoside Rg_1 and Re by frontal analysis chromatography

作　　者：吴秀红[1] 林炳昌[1] 

机构地区：[1]大连理工大学化工学院

出　　处：《化工学报》2007年第9期2270-2275,共6页CIESC Journal

基　　金：国家中小企业创新基金项目(03C26212100852)~~

摘　　要：The determination of isotherm and prediction of retention values are the basis of the optimization of separation conditions of preparative chromatography.In this paper,the isotherms of ginsenoside Rg1 and Re from Panax ginseng were studied.Ginsenoside Rg1 and Re were prepared by chromatography column from Panax ginseng raw material,and the isotherms of ginsenoside Rg1 and Re were determined by frontal analysis(FA) on spherical C18 solid phase.The adsorption parameters G and b were set based on the competitive Langmuir model.In the mean time,the retention time of ginsenoside Re was obtained with the results of nonlinear chromatography theory.The calculated retention time were compared with that of experimental results with average relative error 3.68%.Additionally,some problems related to fitting based on the competitive Langmuir model were found and discussed.The determination of isotherm and prediction of retention values are the basis of the optimization of separation conditions of preparative chromatography. In this paper, the isotherms of ginsenoside Rgl and Re from Panax ginseng were studied. Ginsenoside Rgl and Re were prepared by chromatography column from Panax ginseng raw material, and the isotherms of ginsenoside Rgl and Re were determined by frontal analysis （FA） on spherical C18, solid phase. The adsorption parameters G and b were set based on the competitive Langmuir model. In the mean time, the retention time of ginsenoside Re was obtained with the results of nonlinear chromatography theory. The calculated retention time were compared with that of experimental results with average relative error 3.68%. Additionally, some problems related to fitting based on the competitive Langmuir model were found and discussed.

关 键 词：吸附等温线 迎头法 非线性色谱 双组分 竞争Langmuir 人参皂苷RG1 人参皂苷RE 

分 类 号：O658[理学—分析化学]