梗阻性黄疸围手术期肾髓质水通道蛋白2表达变化的实验研究  

Investigation on Perioperative Renal Aquaporin 2 Expression in Experimental Obstructive Jaundice

作　　者：王勇[1] 韩继龙[1] 刘金钢[1] 

机构地区：[1]中国医科大学盛京医院第二胆道微创外科,沈阳110004

出　　处：《中国普外基础与临床杂志》2007年第5期538-542,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：辽宁省教育厅基金资助(编号:2004D282)~~

摘　　要：目的探讨大鼠胆道梗阻解除后肾髓质集合管上皮细胞形态学改变及水通道蛋白2(AQP2)表达的变化。方法30只大鼠按随机表法随机分为假手术组(n=10)和梗阻性黄疸组(n=20)。梗阻性黄疸组大鼠全麻后建立梗阻性黄疸模型;假手术组大鼠行假手术。7d后再次全麻解除胆道梗阻,而假手术组再次行假手术。各组动物分别于解除梗阻后立即或24h处死取材。将静脉血离心后血清冻存备检肝、肾功能;部分肾脏标本分离后冻存,Western blot法半定量检测AQP2表达;部分肾脏固定后作光镜观察。结果各组动物均顺利完成实验,无实验动物死亡。梗阻性黄疸组大鼠取材时见腹腔较多金黄色腹水。解除梗阻后24h大鼠血清胆红素水平下降〔(45.95±8.39)μmol/L〕,P<0.01,血肌酐及尿素氮未见明显改变。梗阻性黄疸组肾髓质AQP2表达(15665.30±1181.85)较假手术组(21966.20±1544.70)明显下降(P<0.01),解除梗阻后梗阻性黄疸组肾髓质AQP2表达增强(19490.80±4239.32),P<0.01。结论胆道梗阻会造成肾髓质集合管上皮细胞结构损伤,同时下调AQP2表达。Objective To investigate the changes of renal medulla aquaporin 2 expression and morphological changes of epithelia of collecting tube after bile duct recanalizaiton operation. Methods Thirty rats were divided into two groups randomly. Common bile duct ligation was performed on 20 experimental rats with silicon tubes 2 mm in extre-diameter, and sham operation on the other 10 rats. Seven days later, bile duct recanalizaiton was performed on obstructive jaundice group and sham operation on contrast group. Experimental rats were divided into two subgroups randomly. Half of them were killed immediately and the others would be killed 24 hours later. Serum of each rat was collected to detect hepatic function and renal function. Renal medulla was fixed for microscopic examination and was kept in the-80℃ refrigerator for aquaporin 2 expression measurement by Western blot technique. Results All of the animals accomplished the experiment smoothly. Golden ascites were found in the rats of obstructive jaundice group. Twenty-four hours after recanalization, serum bilirubin levels decreased （（45.95±8.39） μmol/L], P〈 0.01, and there was no significant change in blood urine and creatine level. Compared with sham operation group （21 966.20±1 544.70）, expression of aquaporin 2 decreased significantly after common bile duct ligation in obstructive jaundice group （15 665.30±1 181.85）, P〈0.01. After recanalizaion, the expression of aquaporin 2 in obstructive jaundice group increased （19 490.80 ± 4 239. 32）, P〈0. 01. Conclusion Common bile duct obstruction would lead to epithelium injury of renal collecting tube, and down regulate the aquaporin 2 expression.

关 键 词：梗阻性黄疸 水通道蛋白2 围手术期 

分 类 号：R657.4[医药卫生—外科学]