大豆根际土壤中氢氧化细菌的分离、筛选和基本特征  

Isolation,screening and characterization of hydrogen-oxidizing bacteria in soybean rhizosphere

作　　者：陈兴都[1] 王卫卫[1] 郭利伟[1] 熊本涛[1] 付博[1] 

机构地区：[1]西北大学生命科学学院,西安710069

出　　处：《应用生态学报》2007年第9期2069-2074,共6页Chinese Journal of Applied Ecology

基　　金：西北大学科研启动基金项目;陕西省自然科学基金资助项目(2002C113).

摘　　要：土壤中的氢氧化细菌能够利用土壤中的H2为能源并同化CO2,增加其种群数量并促进作物生长.采用气体循环培养体系(持续通氢气装置),通过电解水的方式产生H2,与通入的空气混合,形成流速为280ml.min-1、含H2量为41.6～125μmol.L-1的混合气体.采用矿质盐固体培养基,在适当的H2、O2和CO2下以H2作为唯一能源分离氢氧化细菌.采用此方法从大豆根际土壤样品中分离出40余株细菌,对其进行耗氢能力测定表明,有20株菌具有氧化氢功能和自养生长能力,初步判断这20株菌为氢氧化细菌,并测定了菌落形态及生理生化特征.Soil hydrogen-oxidizing bacteria can utilize soil gaseous hydrogen as an energy source to assimilate carbon dioxide. In this paper, a gas-cycle incubation system （H2 treatment system） which can provide stable H2 flow （41.6-125 μmol · L^-1） via H20 electrolysis was developed, and a mineral salt agar medium （MSA） was used to separate the hydrogen-oxidizing bacteria in soybean rhizosphere. A total of 40 bacterial strains were isolated from the incubation system, and their hydrogen uptake was measured. 20 of the strains could utilize hydrogen and grow autotrophically, which were preliminarily identified as hydrogen-oxidizing bacteria. Their colony form and physiological-biochemical characteristics were studied.

关 键 词：氢氧化细菌 土壤 气体循环培养体系 筛选 

分 类 号：S565.1[农业科学—作物学] S154.3