土壤低剂量芘污染对蚯蚓若干生化指标的影响  

Effects of low dosage pyrene pollution on biochemical characters of earthworm (Eisenia fetida) in soil

作　　者：张薇[1] 宋玉芳[1] 孙铁珩[1] 刘淼[1] ML Ackland Belogolova Galina 

机构地区：[1]中国科学院沈阳应用生态研究所陆地生态过程重点实验室 [2]Centre for Cellular & Molecular Biology,School of Life and Environmental Sciences,Deakin University,221 Burwood Highway,Burwood,Melbourne,Victoria 3125,Australia [3]Vinogradov Institute of Geochemistry SB RAS,P.O.Box 4019,Irkutsk-33,664033,Russia

出　　处：《应用生态学报》2007年第9期2097-2103,共7页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划项目(2004CB418503);国家自然科学基金项目(20577056;20337010);中-俄自然资源与生态环境联合研究中心及中-俄合作研究(NSFC-RFBR)资助项目(20611120015)

摘　　要：通过人工污染土壤的方法,设计芘的暴露浓度为0、60、120、240、480、960μg.kg-1.暴露实验进行1、3、7和14d后,分别检测蚯蚓内脏中细胞色素P450含量、谷胱甘肽转移酶(GST)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量.结果表明,在供试浓度范围内,蚯蚓内脏中各生化指标对污染物暴露指示的敏感性存在差异:其中P450含量、GST和SOD活性最为敏感;POD和CAT活性次之;而MDA含量未对低剂量的芘暴露起到明显的指示作用.研究同时发现,低剂量污染物暴露的时间效应要强于剂量效应的影响.因而,在进行生态毒性诊断时,采用多指标和多时段的检测对增强指示的灵敏性和有效性尤为重要.By the method of artificial soil pollution, an exposure experiment with different concentrations of pyrene （0, 60, 120, 240, 480, 960 μg · kg^-1 ） was conducted to determine the cytochrome P450 and MDA contents and the glutathione-S-transferase （ GST）, superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） activities in earthworm gut after exposure for 1, 3, 7 and 14 days. The results indicated that within the range of test pyrene concentrations, all the biochemical indices tested differed in their sensitivity to pyrene toxicity, among which, P450 content and GST and SOD activities were most sensitive, followed by POD and CAT activities, while MDA content did not show any obvious response. Exposure duration had stronger effects than exposure dosage. In diagnosing the ecotoxicity of soil pollutant, it could be necessary to use a combined multi-time and multi-index diagnostic method to enhance the sensitivity and effectiveness of the indices adopted.

关 键 词：芘 低剂量 蚯蚓 细胞色素P450 谷胱甘肽转移酶 抗氧化酶 生物标记物 

分 类 号：X53[环境科学与工程—环境工程]