重组近交系群体定位绿豆抗绿豆象基因  

Mapping of Genes Resistant to Bruchid in Mungbean Using Recombinant Inbred Lines Population

作　　者：梅丽[1] 王素华[1] 王丽侠[1] 刘长友[1] 孙蕾[1] 徐宁[1] 刘春吉[2] 程须珍[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]澳大利亚联邦科学与工业研究组织植物研究所

出　　处：《作物学报》2007年第10期1601-1605,共5页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30170635);农业部作物种质资源保护项目(NB04-22-12);国家科技基础条件平台项目(2004DKA30380-08);国家"十一五"科技支撑计划项目(2006BAD02B08)

摘　　要：绿豆象是对绿豆危害最严重的仓储害虫,检测和利用抗绿豆象基因是控制该害虫最经济有效的方法。本研究利用高感豆象绿豆栽培种Berken和高抗豆象绿豆野生种ACC41亚种内杂交得到的重组近交系(RIL)群体及据此构建的1个包含79个RFLP分子标记的遗传图谱,通过对3个种植环境条件下收获的RIL群体进行2年的室内人工接虫鉴定,评价其抗绿豆象表现。QTL作图结果表明,在2年3点试验中在第9连锁群mgM213～VrCS161标记之间均检测到1个QTL,贡献率在74.05%～79.27%,是抗绿豆象主效基因。该QTL的加性效应值为负,表明来自父本ACC41的这个位点的等位基因可以提高绿豆对绿豆象的抗性。本研究结果对开展绿豆抗绿豆象育种以及抗绿豆象基因的精细定位和克隆有重要意义。Mungbean [Vigna radiata（L.） Wiclzek] is one of the main edible beans in China while bruchid are stored pests of grain legumes causing considerable loss to mungbean.The most serious bruchid beetle in Asian is Callosobruchus chinensis（L）.Breeding for bruchid-resistant cultivars is a major goal in mungbean improvement.In this study,we analyzed the QTLs,using a Recombinant-inbred-lines（RIL） population from the cross between a highly susceptible cultivar Berken and a highly resistant wild type ACC41.The RIL population together with their parents were planted in three sites（Australia,Beijing and Nanjing） and evaluated for their resistance by innoculated with C.chinensis at 2005 and 2006.We set the combination of a plant site and a year as one environment,so there are four environments（Australia 2005,Australia 2006,Beijing 2006,and Nanjing 2006）.Chi-square analysis（χ2） showed that except for the population planted in Beijing and evaluated in 2006,the resistance in other three environments supported the 1：1 segregation ratio.This indicated there was a major resistant gene in ACC41.A genetic linkage map with 79 RFLP markers was constructed.Software WinQTLCart was applied to detect QTLs.As a result,a QTL named brI on group 9 steadily existed in the four environments and LOD value was 12.99 to 115.12,explaining 74.05 to 79.27% of the phenotypic variations.It is a major QTL for bruchid resistance.The additive effect of the QTL was negative,indicating that the allele of the loci from ACC41 promotes the resistance.It will be helpful for to bruchid-resistant breeding and plays an important role in mapping and cloning of the bruchid-resistant genes.

关 键 词：绿豆 绿豆象 RIL群体 QTL定位 

分 类 号：S522[农业科学—作物学]