Ⅲ型胶原α链基因mRNA的表达与舌苔形成的关系研究  

Relationship between the expression of collagen3A1 mRNA and the formation of tongue furs

作　　者：张春兵[1] 范媛[2] 詹瑧[3] 张军峰[3] 汪红[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]江苏省口腔医院,江苏南京210029 [3]南京中医药大学基础医学院,江苏南京210029

出　　处：《检验医学》2007年第5期524-527,共4页Laboratory Medicine

基　　金：国家自然科学基金资助项目(30472123&30070910)

摘　　要：目的研究Ⅲ型胶原α链基因表达水平与舌苔形成之间的关系。方法应用基因芯片技术和实时荧光定量逆转录-聚合酶链反应(RT-PCR)技术,检测Ⅲ型胶原α链在不同舌苔中的表达水平。结果基因芯片显示,常见舌苔中Ⅲ型胶原α链表达水平差异有统计学意义;实时荧光定量PCR证实从高到低依次为:黄厚苔>白薄苔>白厚苔>无苔>黄薄苔>胎儿白薄苔。结论常见舌苔中,Ⅲ型胶原α链表达水平有差异,提示Ⅲ型胶原α链基因表达水平的调控在舌苔形成过程中可能有一定的意义。Objective To investigate the relationship between the expression of collagen3A1（COL3A1） mRNA and the formation of different tongue furs. Methods Total RNA of tongue epithelial tissue was prepared and detected by cDNA microarray.The COL3A1 mRNA was measured by real-time quantitative reverse transcription polymerase chain reaction（RT-PCR）. Results The COL3A1 mRNA expression was different in diverse tongue furs,from yellow thick fur,white thin fur,white thick fur,no-fur,yellow thin fur to fetal white thin fur with the tendency to decrease. Conclusions The expression of COL3A1 mRNA may be an important event in the formation of the tongue fur.

关 键 词：Ⅲ型胶原α链 舌苔 基因芯片 实时荧光定量逆转录-聚合酶链反应 

分 类 号：R446.1[医药卫生—诊断学]