左旋肉毒碱对人成骨细胞增殖和凋亡的影响  

Effects of L-carnitine on the proliferation and apoptosis in human osteoblasts

作　　者：崔蓉蓉[1] 谢辉[1] 黄佼[1] 袁凌青[1] 彭依群[1] 周后德[1] 罗湘杭[1] 廖二元[1] 

机构地区：[1]中南大学湘雅二医院内分泌代谢研究所,长沙410011

出　　处：《中国骨质疏松杂志》2007年第10期681-685,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目(30600661)

摘　　要：目的观察左旋肉毒碱（L-carnitine，L-C）对人成骨细胞增殖和凋亡的作用。方法以^3H掺人法（^3H—TdR）测定左旋肉毒碱对人成骨细胞增殖的影响；以吖啶橙，溴化乙啶染色和细胞凋亡ELISA（酶联免疫吸附法）试剂盒测定左旋肉毒碱对人成骨细胞凋亡的影响；以免疫印记法检测左旋肉毒碱干预前后活化型Caspase-3、-9蛋白质表达水平的改变。结果10～100mmol／L左旋肉毒碱能促进人成骨细胞增殖。吖啶橙，溴乙啶染色法示100mmol／L左旋肉毒碱干预使人成骨细胞凋亡细胞明显减少。ELISA结果示10～100mmol／L左旋肉毒碱干预可显著抑制人成骨细胞凋亡。10～100mmol／L左旋肉毒碱干预可抑制无血清饥饿诱导的人成骨细胞caspase-3、9活化增加。结论左旋肉毒碱促进人成骨细胞增殖，并通过下调Caspase-3、-9的活化抑制人成骨细胞凋亡。Objective To observe the effect of L-carnitine （L-C） on the proliferation and apoptosis in human osteoblasts （hOB）. Methods The effect of L-C on the proliferation of hOB cells was assessed by [ ^3H ] thymidine incorporation. The effect of L-C on the apoptosis of hOB cells was analyzed by acridine orange/ethidium bromide staining and Cell Death Detection ELISA Kit. The protein active caspase-3 ,-9 was determined by Western blot analysis. Results Treatment with 10 - 100 mmol/L concentration of L-C to hOB cells promoted cell proliferation. 10- 100 mmol/L L-C suppressed serum deprivation-induced hOB apoptosis. Western blot analysis confirmed that 10 - 100 mmol/L L-C reversed the serum deprivation-induced caspase-3 ,-9 activation in hOB cells. Conclusions L-C promoted the cell proliferation in human osteoblasts. L-C suppressed the cell apoptosis in human osteoblasts through the down-regulation of caspase-3,-9 activation.

关 键 词：左旋肉毒碱 增殖 凋亡 人成骨细胞 

分 类 号：R274.91[医药卫生—中西医结合]