八肽胆囊收缩素对脂多糖攻击小鼠抗炎症细胞因子表达的影响  

Cholecystokinin octapeptide augments expression of anti -inflammatory cytokines in mice challenged by lipopolysaccharide

作　　者：倪志宇[1] 闫玉仙[1] 丛军 徐锦荣[1] 高峰[1] 李淑瑾[1] 马春玲[1] 丛斌[1] 

机构地区：[1]河北医科大学基础医学院,石家庄050017 [2]河北赵县医院,051530

出　　处：《中国危重病急救医学》2007年第10期580-583,共4页Chinese Critical Care Medicine

基　　金：国家自然科学基金资助项目（30270529）;河北省科技厅博士基金资助项目（06547008D）

摘　　要：目的观察脂多糖（LPS）攻击小鼠白细胞介素-4（IL-4）、IL-10的动态变化规律，以及八肽胆囊收缩素（CCK-8）对其表达的影响。方法随机将小鼠分组，每组7只。腹腔注射LPS10mg／kg，于攻击后0、2、4、6和12h检测血清、肺组织IL-4和IL-6表达高峰的时间。对照组腹腔注射生理盐水0．2ml；LPS组腹腔注射LPS10mg／kg；LPS＋CCK-8组在注射LPS前30min腹腔注射CCK-8 60μg／kg；CCK-8组腹腔注射CCK-8 60μg／kg。用酶联免疫吸附法（ELISA）及逆转录-聚合酶链反应（PT—PCR）方法检测各组小鼠血清和肺组织中IL-4、IL-10的含量及其mRNA的表达情况。结果LPS攻击后2h血清及肺组织IL-4、IL-6均显著升高，血清IL-4、IL-6于4h和6h达高峰；肺组织IL-4、IL-6则均于6h达高峰。说明LPS攻击可使小鼠血清及肺组织中IL-4、IL-10的蛋白及mRNA表达均增加；预先注入CCK-8可使IL-4和IL-10的蛋白以及mRNA表达均进一步增加（P均〈O．01）；而单独注射CCK-8对血清、肺组织IL-4、IL-10的表达无明显影响。结论CCK-8可能通过进一步增加LPS攻击小鼠IL-4、IL-10的表达参与抗炎反应过程，从而减轻LPS引起的肺组织炎症反应。Objective To observe the effects of cholecystokinin octapeptide （CCK - 8） on the expression of anti -inflammatory cytokines interleukin - 4 （IL - 4） and IL - 10 in lipopolysaccharide （LPS） challenged mice. Methods Kunming mice were randomly assigned into four groups （each n = 7）： ①Intraperitioneal injection of LPS （10 mg/kg）, and the times of peak level of IL - 4 and IL - 6 expression in serum and lung tissue were noted at 0, 2, 4, 6 and 12 hours after challenge. ② Control group （intraperitioneal injection of normal saline 0.2 ml）. ③LPS＋CCK -8 group （intraperitioneal injection of CCK-8 60 μg/kg 30 minutes before giving LPS）. ④CCK - 8 group （intraperitioneal injection injection CCK- 8 60 μg/kg） at different time points. The expressions of IL - 4 and IL - 10 in the serum and lung tissues were assayed by enzyme linked immunoadsorbent assay （ELISA） and reverse transcription - polymerase chain reaction （RT-PCR）. Results Two hours after LPS challenge, IL - 4 and IL - 6 were increased significantly in serum and lung tissue. At 4 hours and 6 hours, serum IL - 4 and IL - 6 reached their peak levels, while in lung tissue they reached their peak levels at 6 hours. Pre - treatment of CCK - 8 augmented IL - 4 and IL - 10 expressions in LPS challenged mice （both P^0.01）. But single CCK - 8 injection showed no significant effect on IL - 4 and IL - 10 in serum and lung tissue. Conclusion CCK - 8 is involved in the anti -inflammatory response by increasing the expressions of IL - 4, IL - 10 in LPS challenged mice, and in turn it alleviates the inflammatory response in lung tissue.

关 键 词：缩胆囊素 脂多糖 白细胞介素-4 白细胞介素-10 

分 类 号：R686[医药卫生—骨科学]