蛇油中游离脂肪酸成份分析及其体外淬灭单线态氧能力的研究  

Study of Free Fatty Acids in Snake Oil and of Ability to Quench Singlet-Oxygen in Vitro

作　　者：刘一唯[1] 杨成对[2] 陈培榕[2] 周华峰[1] 段明星[1] 

机构地区：[1]生物膜与膜生物工程国家重点实验室清华大学生物科学与技术系,北京100084 [2]清华大学分析中心,北京100084

出　　处：《现代科学仪器》2007年第4期96-99,共4页Modern Scientific Instruments

摘　　要：本文以取自蝮蛇和乌梢蛇的混合蛇油为检材,采用气相色谱-质谱法(GC-MS)对其中的游离脂肪酸成分进行了定性定量分析,分析结果表明,蛇油中含有多种的饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸,其中以油酸的含量为最丰;ω-6系列多不饱和脂肪酸与ω-3系列多不饱和脂肪酸的含量比为8,在理想的比值范围内(5 -10之间)。本文还利用微弱发光检测技术对蛇油在体外淬灭单线态氧的能力进行了研究,结果显示蛇油中含有某些天然抗氧化功能的活性成分,在一定浓度范围内具有较明显的抗氧化功能。In this paper, the free fatty acids composition of the mixed snake oil of Agkistrodon and Zaocys was analyzed qualitatively and quantitatively by gas chromatography - mass spectrometry （ GC - MS）. The results showed that large amounts of saturated,monounsaturated and polyunsaturated fatty acids （PUFAs） were contained in the snake oil, among which, the content of oleic acid is the most. And the ratio between omega -6 series of PUFAs and omega -3 series of PUFAs is 8, in an ideal ratio range （between5 - 10）. Snake oil~ ability to quench singlet- oxygen in vitro was detected by Bioluminescence Detection Technology. The results demonstrated that some natural active ingredients with antioxidant function were contained in snake oil, which made it have good antioxidant function in a certain concentration range.

关 键 词：蛇油 游离脂肪酸 色谱-质谱联用仪(GC-MS) 淬灭 单线态氧 

分 类 号：R284[医药卫生—中药学]