高效液相色谱法同时测定人血浆中左卡尼汀、乙酰左卡尼汀和丙酰左卡尼汀的浓度  

Simultaneous Analysis of L-carnitine,Acetyl-L-carnitine and Propionyl-L-carnitine in Human Plasma by HPLC

作　　者：孙永旭[1] 陆丛笑[1] 唐启令[1] 曹玉[2] 薛玉增[3] 万秀霞[2] 王春波[2] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院药学部,山东烟台264000 [2]青岛大学医学院,山东青岛266071 [3]聊城市人民医院,山东聊城252000

出　　处：《中国药学杂志》2007年第18期1425-1428,共4页Chinese Pharmaceutical Journal

摘　　要：目的建立同时测定人血浆中左卡尼汀（LC）、乙酰左卡尼汀（ALC）和丙酰左卡尼汀（PLC）的浓度的方法。方法血浆样品经蛋白沉淀法处理,以氨基蒽衍生化后进样测定。色谱条柱采用HypersilC18柱（4.6mm×200mm,5μm）,流动相为乙腈-0.1mol.L^-1乙酸铵（28∶72）,流速为1.2mL·min^-1,检测波长：Ex248nm,Em418nm。结果LC,ALC和PLC的线性范围分别是5-160,1-32,0.25-8μmol·L^-1。平均方法回收率分别为99.48%,99.94%,98.20%。日内、日间RSD均小于5.83%。结论本实验方法灵敏、稳定、结果准确,操作方便,适用于左卡尼汀、乙酰左卡尼汀和丙酰左卡尼汀血药浓度研究性监测。OBJECTIVE To establish a new sensitive method for the simultaneous determination of L-carnitine （ LC）, Acetyl-L- carnitine（ALC） and Propionyl-Lcamitine （PLC） in Human Plasma by HPLC. METHODS The analytes were extracted by protein precipitation and then precolumn derivatization with l-aminoanthracene （IAA） was performed. The fluorescent derivatives were separated on a Hypersil C is column, and the mobile phase consisted of acetonitrile-0. 1 mol· L^-1 ammonium acetate（28： 72）, the flow rate was 1.0 mL · min^-1. The derivatives were monitored with a fluorescent detector set at 248 nm （excitation wavelength） and 418 nm （emission wavelength）. RESULTS The good linearity was observed over the concentration ranges of 5 - 160 μmol· L ^-1 for LC, 1 - 32 μmol · L^-1 for ALC,0. 25 -8 μmol · L^-1 for PLC. The recoveries of LC,ALC and PLC were 99. 48% ,99.94% and 98. 20% ,respectively. The intra- and inter-day validation were adequate with the RSDs of 5.83% or below. CONCLUSION This method is proved to be stable,accurate and convenient to study the concentrations of LC,ALC and PLC in plasma.

关 键 词：左卡尼汀 乙酰左卡尼汀 丙酰左卡尼汀 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]