RP-HPLC测定不同产地白花丹中白花丹醌的含量  

Determination the Content of Plumbagin in Plumbago zeylanica L.from Different Areas by RP-HPLC

作　　者：刘圆[1] 刘超[1] 

机构地区：[1]西南民族大学少数民族药物研究所,成都610041

出　　处：《中国药学杂志》2007年第18期1429-1430,共2页Chinese Pharmaceutical Journal

基　　金：四川省青年基金项目(07ZQ026-011);四川省科技攻关项目(2005);四川省科技厅应用基础项目(2006);四川省教育厅教育教学改革项目(2005)

摘　　要：目的分析不同产地白花丹药材中白花丹醌含量的差异。方法采用KromasilC18柱（4.6mm×200mm,5μm）,柱温：30℃,流动相：甲醇-水（65∶35）,检测波长：213nm,流速：1mL·min^-1。结果白花丹醌在0.0104-0.3328μg,峰面积与进样量具有良好的线性关系,回归方程为Y=-22.32＋9527.9X,r=0.9999,平均回收率为98.7%。结论本法简便、准确、重复性好,可用于控制白花丹药材质量。OBJECTIVE To determine the Plumbagin in Plumbago zeylanica from different areas. METHODS A RP-HPLC method was established using a kromasil C18 column （4. 6 mm ×200 mm,5μm）. The mobile phase was methanol-water（65： 35）. The flow rate was 1 mL · min^- 1 with UV detection at 213 nm. The column temperature was 30℃. RESULTS The method displayed good linearity within the concentration ranges of 0. 010 4 -0. 332 8 μg （r =0. 999 9）. The average recovery was 98.7%. CONCLUSION This method is simple, accurate, reproducible and suitable for the determination of Plumbagin in P. zeylanica L.

关 键 词：白花丹 白花丹醌 含量 RP-HPLC 

分 类 号：R917[医药卫生—药物分析学]