快中子辐照对枯草芽孢杆菌DNA损伤研究  

Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

作　　者：陈晓明[1] 谭碧生[1] 郑春[2] 张建国[1] 王丹[1] 楚士晋[1] 任正隆[3] 

机构地区：[1]西南科技大学,绵阳621010 [2]中国工程物理研究院核物理与化学研究所,绵阳621900 [3]四川农业大学,雅安625000

出　　处：《高能物理与核物理》2007年第10期972-977,共6页High Energy Physics and Nuclear Physics

基　　金：中国工程物理研究院预研项目(052056);四川省教育厅(2005C13);西南科技大学引进人才项目基金(051103)资助~~

摘　　要：采用脉冲场凝胶电泳技术检测并定量分析了CFBR-Ⅱ快中子脉冲堆产生的快中子在不同剂量和剂量率条件下,对枯草芽孢杆菌黑色变种(ATCC 9372)DNA双链断裂的诱导.通过DNA释放百分比PR值、DNA断裂水平L值、断裂DN A平均分子量和DNA片段分布等指标的分析,结果表明:在不同的辐射条件下,DNA片段均明显分布于两个区域,表明枯草芽孢杆菌黑色变种DNA分子上可能存在对中子辐射较为敏感的位点;并且随着中子辐射剂量和剂量率的变化,DNA释放百分比PR值、DNA断裂水平L值和各片段区双链断裂的含量也会发生一定规律性的变化.The content of Bacillus substilis var. niger （ATCC 9372） DNA double-strand breaks induced by neutron radiation at different doses and different dose rates respectively, was measured and quantified by pulsed-field gel electrophoresis. By the analyses of DNA release percentage （PR）, DNA breakage level （L）, average molecular weight of breakage DNA and DNA fragment distribution, the results showed that DNA fragments were obviously distributed into two regions in the different irradiation condition. This suggestes that there are some sensitive points to neutron radiation in the Bacillus substilis var. niger molecule. The DNA release percentage, DNA breakage level and the content of DNA fragments in different region varied regularly in some extent with change of the neutron radiation dose or dose rate.

关 键 词：DNA双链断裂 脉冲场凝胶电泳 枯草芽孢杆菌黑色变种 CFBR-Ⅱ快中子脉冲堆 辐照 热点 

分 类 号：Q937[生物学—微生物学]