氨氯地平对缺氧和复氧损伤心肌细胞内pH值及Ca^(2+)浓度的影响  

The effect of amlodipine on intracellular pH and calcium concentration in hypoxic and reoxygenated cardiac myocytes

作　　者：张昀昀[1,2] 王军章 鲁陈万春 金正均[1,2] 

机构地区：[1]上海市第六人民医院 [2]上海第二医科大学

出　　处：《中华心血管病杂志》1997年第3期219-220,共2页Chinese Journal of Cardiology

摘　　要：用荧光探针Ｆｕｒａ－２及ＢＣＥＣＦ结合计算机图象处理技术，观察氨氯地平对缺氧和复氧损伤心肌细胞内ｐＨ值和Ｃａ２＋浓度的影响。发现缺氧和复氧损伤细胞内游离钙离子的浓度明显上升，ｐＨ值下降；氨氯地平能显著地降低缺氧损伤的心肌细胞内的游离Ｃａ２＋浓度，并抑制细胞酸化；但对缺氧损伤后再复氧的心肌细胞内游离Ｃａ２＋的浓度和ｐＨ值均无显著影响。Fluorescent probes combined with digital image processing technique were used to study the effect of amlodipine on intracellular pH and calcium concentration in hypoxic and reoxygenated cardiac myocytes. The results indicated that the calcium concentration significantly increased while the pH decrased during hypoxia and reoxygenation. Amlodipine could significantly reduce free calcium concentration and inhibit intracellular acidosis during hypoxia. However, it had no effect on variations of both pH and calcium concentration induced by reoxygenation.

关 键 词：心肌缺血 氨氯地平 缺氧 复氧 心肌细胞 PH 钙 

分 类 号：R972[医药卫生—药品] R542.205[医药卫生—药学]