Au标蛋白自组装表面增强拉曼光谱的研究  

Surface Enhanced Raman Spectroscopic Study on the Gold-Labeled Protein Self-Assembled Surface

作　　者：朝克夫[1] 张友林[1] 孔祥贵[1] 冯力蕴[1] 李冰[1] 曾庆辉[1] 宋凯[1] 孙雅娟[1] 

机构地区：[1]中国科学院长春光学精密机械与物理研究所激发态重点实验室

出　　处：《光谱学与光谱分析》2007年第9期1757-1760,共4页Spectroscopy and Spectral Analysis

基　　金：中国科学院重大创新项目(Kjcx2-sw-h12-02);荷兰皇家科学院与中国科学院交流项目;国家自然科学基金项目(10674132;60601015)资助

摘　　要：采用免疫Au标技术,用尺寸大约在13 nm的Au胶体颗粒标记了人血清蛋白(Human IgG),然后将Au标蛋白复合体固定在通过三氨基三乙氧基硅烷和戊二醛自组装单膜的Si片上。这种方法在基底表面上不仅牢固地固定了单层Au纳米颗粒标记的蛋白分子复合体而且提高了复合体的表面覆盖度,保持了生物分子的结构。利用原子力显微镜(AFM)观察了Au标蛋白的自组装表面。实验结果表明Au标蛋白在Si片表面聚积形成一定的Au标蛋白分子的复合体"岛状"单层,并且在这些岛状单层上获得了很明显的标记蛋白的表面增强拉曼散射(SERS)信号。文章在Si表面自组装了Au标蛋白分子,获得了较好的蛋白分子的SERS信号,提供了一种研究蛋白分子的SERS活性基底。The human IgG molecules were labeled with 13 nm gold nanoparticles and the complex of the gold-labeled human IgG molecules was immobilized on a silicon surface modified by 3-aminopropyltriethoxysilane and glutaraldehyde.The method increases not only the tightness but also the surface coverage for immobilization of the complex and retains protein configuration well on the silicon surface.The self-assembled complex surface was observed by AFM.The complex aggregated on the silicon surface and the ＂island＂ monolayer of the complex was obtained.Meanwhile the SERS spectrum of the complex self-assembled ＂island＂ monolayer on silicon surface was presented.In the present paper,the gold labeled human IgG molecules were self-assembled on the silicon surface,SERS spectra of protein were obtained and as SERS active substrates were provided for the study of the protein molecules.

关 键 词：表面增强拉曼散射 Au标蛋白 自组装 

分 类 号：O657.3[理学—分析化学]