SOD活性增高的拟南芥晚花突变体具有增强的非生物胁迫耐受性  

Arabidopsis Late-flowering Mutants with Elevated SOD Activities Show Enhanced Tolerance to Abiotic Stresses

作　　者：汪本勤[1] 陈曦[1] 向成斌[1] 

机构地区：[1]中国科学技术大学生命科学学院

出　　处：《植物学通报》2007年第5期572-580,共9页Chinese Bulletin of Botany

摘　　要：为了研究晚花突变表型和非生物胁迫耐受性之间是否存在相关性,以遗传背景同为Wassilewskija(Ws-2)的7个拟南芥(Arabidopsis thaliana L.)晚花突变体(CS2235、CS2238、CS2239、CS2240、CS2246、CS2248和CS6208)为材料,通过干旱胁迫、盐胁迫和氧化胁迫实验,发现晚花突变体的耐旱、耐盐和耐氧化性都较野生型(wildtype,WT)强。对叶片失水速率和超氧化物歧化酶(superoxide dismutase,SOD)活性测定结果也表明,晚花突变体的保水能力和SOD活性均高于野生型。相关性回归分析表明SOD活性与耐氧化性、耐旱性、耐盐性之间呈正相关,耐旱性和耐盐性随耐氧化性增强而增强。实验结果表明,这些晚花突变体的表型和非生物胁迫耐受性之间存在相关性。To study whether a correlation exists between late-flowering phenotype and abiotic stress tolerance, seven Arabidopsis late-flowering mutants with the same genetic background of Wassilewskija ecotype were tested for tolerance to drought, salt, and oxidative stress. These mutants displayed significantly higher tolerance to the stresses tested than the wildtype（WT）. In addition, the rate of water loss from detached leaves and superoxide dismutase（SOD） activity were also compared between the WT and the mutants. It was found that the mutants showed a much better water conservation and higher superoxide dismutase activity than the WT. A positive correlation was found between SOD activity and abiotic stress tolerance, suggesting that oxidative stress tolerance plays an important role in abiotic stress tolerance.

关 键 词：拟南芥 耐旱性 晚花突变体 耐氧化性 耐盐性 SOD活性 

分 类 号：Q945.78[生物学—植物学]