利用SSR分子标记对鹅掌楸自由授粉子代的父本分析  

The Paternity Analysis for Open-pollination Progenies of Liriodendron L. Using SSR Markers

作　　者：孙亚光[1] 李火根[1] 

机构地区：[1]南京林业大学林木遗传与基因工程重点实验室,南京210037

出　　处：《植物学通报》2007年第5期590-596,共7页Chinese Bulletin of Botany

基　　金：江苏省自然科学基金(No.BK2006211)

摘　　要：利用自行开发的12个EST-SSR分子标记,采用最大似然法对鹅掌楸(L.chinense (Hemsl.)Sarg.)实验群体3个半同胞家系的180个子代进行父本分析。结果表明,每个SSR位点的等位基因数为3-7,平均为4.67;其平均观测杂合度(Ho)、平均期望杂合度(He)及平均多态信息含量(PIC)分别为0.458、0.635和0.580。利用12个SSR标记可在95%的可信度确定114个子代的父本,占子代群体的63.3%,其累积排除概率为98.52%。自由授粉状态下,鹅掌楸的自交率为11.6%,而北美鹅掌楸自交率为0,且种内交配比例大于种间交配。鹅掌楸平均有效花粉散布距离为20-30m,最大散布距离为70m。The patterns of effective pollen dispersal were explored in an experimental population of Liriodendron L. Paternities of 180 open-pollination progenies from 3 maternal trees were identified by twelve nuclear SSR polymorphic loci from expressed sequence tags of Liriodendron A software, CERVUS, was used to assign the paternity of each progeny based on the method of maximum likelihood analysis. The number of alleles per locus ranged from 3 to 7, with average of 4.67. The observed and expected heterozygosities （Ho and He） ranged from 0.263 to 0.680 and from 0.476 to 0.748, with averages of 0.458 and 0.635, respectively. The polymorphic information content （PIC） ranged from 0.428 to 0.704, with average of 0.580. The cumulative exclusion probability of 12 SSR loci was 98.52%. Among 180 progenies, 114 （accounting for 63.3%） progenies could be assigned paternity at strict confidence level of 95%, the remained 66 （accounting for 36.7%） progenies could also be assigned paternity at relaxed confidence level （80%）. The selfing rate of open-pollinated L. chinense Sarg. was 11.6%, while the selfing rate of L. tulipifera L. was zero. It seems that intraspecies mating occurred more frequently than interspecies mating in Liriodendron. The average effective pollen dispersal distance of Liriodendron ranged from 20 m to 30 m, with the maximum dispersal distance of 70 m.

关 键 词：鹅掌楸 交配方式 父本分析 花粉流 SSR 

分 类 号：Q943[生物学—植物学]