芨芨草愈伤组织器官发生及植株再生  

In vitro Regeneration of Achnatherum splendens (Trin.) Nevski

作　　者：王瑛[1] 周燕[1] 高述民[1] 梁蕊[1] 刘艳[1] 李莉[1] 陈新德 

机构地区：[1]北京林业大学生命科学与技术学院,北京100083 [2]新疆石河子农八师农业局,石河子832000

出　　处：《植物学通报》2007年第5期636-641,共6页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30671709);北京林业大学林木;花卉遗传育种教育部重点实验室课题(No.05-05)

摘　　要：芨芨草(Achnatherum splendens (Trin.) Nevski)种子消毒并在MS培养基上萌发获得无菌苗,以幼苗的叶鞘和胚轴为外植体诱导愈伤组织,经继代后进一步诱导不定芽及生根。研究结果表明,诱导愈伤组织最适合的培养基为B5+1.5mg.L-12,4-D+0.5mg.L-1NAA;诱导芽分化较适合的培养基为B5+0.5mg.L-16-BA+0.2mg.L-1NAA;1/4B5+1.0mg.L-1NAA+0.2mg.L-1IBA+1.0g.L-1活性炭培养基则有利于芨芨草试管苗的生根。本实验建立了完整的芨芨草植株再生体系,移栽成活率高。We studied the regeneration of Achnatherum splendens （Trin.） Nevski in vitro and selected several favorable media. Germ-free plants were produced with germination on MS medium. Callus was induced with B5＋1.5 mg·L^-1 2,4-D＋0.5 mg·L^-1 NAA, with leaf sheath and hypocotyls as explants. After two subcultures, shoots and roots were induced. The medium containing B5＋ 0.5 mg·L^-16-BA ＋ 0.2 mg·L^-1NAA was suitable for shoot induction. For root induction, the medium containing 1/4 B5＋1.0 mg·L^-1 NAA＋ 0.2 mg·L^-1 IBA ＋1.0 g·L^-1 active carbon was favorable. With this regeneration system for A. splendens （Trin.） Nevski, we achieved the highest survival rate of transplants.

关 键 词：芨芨草 愈伤组织 器官发生 再生植株 

分 类 号：Q943.1[生物学—植物学]