HLA半相合非清髓性造血干细胞与间充质干细胞共移植治疗重症再生障碍性贫血1例  

Treatment of severe aplastic anemia with cotransplantation of HLA haploidentical peripheral blood stem cells and mesenchymal stem cells:one case report

作　　者：白海[1] 王存邦[1] 葸瑞[1] 吴涛[1] 张茜[1] 徐淑芬[1] 王晓靖[1] 路继红[1] 周进茂[1] 

机构地区：[1]兰州军区兰州总医院全军血液病中心,兰州730050

出　　处：《临床血液学杂志》2007年第5期291-292,共2页Journal of Clinical Hematology

基　　金：全军"十一五"杰出人才基金资助项目(No:6J005)

摘　　要：目的:观察HLA半相合非清髓性造血干细胞与间充质干细胞(MSC)共移植治疗重症再生障碍性贫血(SAA)的疗效及安全性。方法:1例24岁男性SAA患者。应用非清髓性预处理方案,进行HLA半相合异基因外周血造血干细胞和MSC共移植。移植rhG-CSF动员的供者外周血单个核细胞9.22×108/kg,CD34+细胞8.56×106/kg,及体外扩增培养的供者骨髓MSC2.12×105/kg。结果:移植后+12d中性粒细胞数>0.5×109/L,+21d WBC4.5×109/L,Hb99g/L,PLT108×109/L。经HLA配型,红细胞亚型和VNTR检测,为供者型完全嵌合体。随访14个月,无急、慢性移植物抗宿主病(GVHD)发生。结论:HLA半相合非清髓性造血干细胞与MSC共移植治疗SAA是安全有效的方法。Objective：To explore the feasibility of blood stem cells and mesenchymal stem cells （MSC） from HLA haploidentical sibling cotransplantation for severe aplastic anemia （SAA）. Method： A 24 years old patient with SAA was transplantated with 9.22 ×10^8/kg peripheral blood mononuclear cells and 8.56×10^6/kg CD34^＋ cells and 2.12 ×10^5/kg MSC from HLA haploidentieal sibling. Conditioning regimen was nonmyeloablative. Resalt： Neutrophil amount 〉0.5×10^9/L was found at day 12 after transplantation, at day 21 white blood cells was 4.5×10^9/L,hemoglobin was 99g/L, platelet was 108 ×10^9/L. Examination of HLA, blood type and VNTR revealed a complete chimerism in the patient. There was no aGVHD and cGVHD during 14 months. Conclasion： Nonmyeloablative blood stem cells and MSC cotransplantation from HLA haploidentical sibling for severe aplastic patients is salty and reliable.

关 键 词：贫血 再生障碍性 异基因外周血造血干细胞移植 间充质干细胞移植 HLA半相合 非清髓性预 处理 

分 类 号：R556[医药卫生—血液循环系统疾病]