安速快速检测试剂盒诊断幽门螺杆菌的现症感染  

Evaluation of the Assure H pylori Rapid Test for detection of Helicobacter pylori

作　　者：杨桂彬[1] 魏红[1] 胡伏莲[2] 牟方宏[1] 崔梅花[1] 

机构地区：[1]北京大学航天临床医学院消化科,北京市100049 [2]北京大学第一医院消化科,北京市100034

出　　处：《世界华人消化杂志》2007年第23期2545-2548,共4页World Chinese Journal of Digestology

摘　　要：目的:评价安速幽门螺杆菌(H pylori)快速检测卡诊断H pylori现症感染的敏感性及特异性.方法:对154例因上消化道症状进行胃镜检查的患者同时进行快速尿素酶试验及Warthin- Starry银染,同时取指血进行安速快速检测试剂盒检测.以快速尿素酶试验和Warthin-Starry银染同时阳性或阴性作为诊断是否H pylori感染的金标准.结果:和金标准相比较,安速快速检测试剂盒敏感性为92.0%(81/88),特异性为94.0%(47/50).准确率为92.8%(128/138);与RUT相比安速快速检测试剂盒敏感性为89.7%(87/97),特异性为91.2%(52/57),准确性为90.3%(139/154).结论:安速快速检测试剂盒在没有进行根除治疗的患者中能够较好的反应现症感染的情况.临床可用于H pylori现症感染的诊断.AIM： To evaluate the accuracy of a new officebased serological test （Assure H pylori Rapid Test with current infection marker） for the detection of current infection with Helicobacter pylori, compared with the Warthin-Starry stain and rapid urease test （RUT）. METHODS： Warthin-Starry staining and RUT for detection of current infection with H pylori was performed in 154 consecutive patients with upper gastrointestinal symptoms who underwent gastroscopy. The combination of the results of RUT and Warthin-Starry is regarded as the gold standard for detection of current infections with H pylori. All subjects simultaneously underwent serological testing with the Assure H pylori Rapid Test kit, using a 20 μL finger prick blood sample. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of this test were calculated against those of the RUT and Warthin-Starry standard. RESULTS： Compared to the gold standard,sensitivity and specificity of this new serological test were 92.0% （81/88） and 94.0% （47/50）, respectively. The positive and negative predictive values of this test were 96.4% （81/84） and 87.0% （47/54）, respectively, and accuracy was 92.8% （128/138）. Compared to RUT, sensitivity and specificity of this new serological test were 89.7% （87/97） and 91.2% （52/57）, respectively. The positive and negative predictive values were 94.6% （87/92） and 83.9% （52/62）, respectively, and accuracy was 90.3% （139/154）. CONCLUSION： The Assure H pylori Rapid Test, like RUT, is a simple and accurate method for rapid diagnosis of current infection with H pylori .

关 键 词：幽门螺杆菌 血清学 诊断 

分 类 号：R446.5[医药卫生—诊断学]