乙型肝炎病毒前S1抗原与HBV DNA及肝功能的关系研究  

Study on Correlation between HBV PreS1 antigen and HBV DNA and Liver Function

作　　者：刘贵育[1] 肖春红[1] 

机构地区：[1]安徽医科大学第一附属医院,合肥230022

出　　处：《临床输血与检验》2007年第4期327-329,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的探讨不同模式乙肝患者血清中乙肝病毒前S1抗原(HBV PreSl-Ag)与HBV DNA及ALT、AST的相互关系,分析HBV PreSl-Ag用于乙肝临床诊断的意义。方法采用酶联免疫吸附实验(ELISA)检测350例乙肝患者血清HBV PreSl-Ag,荧光定量PCR检测HBV DNA,全自动生化分析仪检测ALT和AST。结果相同乙肝模式患者血清HBVPreSl-Ag与HBV DNA检出率的差异无显著性;HBVPreSl-Ag与HBV DNA、HBeAg与HBVPreSl-Ag、HBeAg与HBV DNA的符合率分别为86.9%(K=0.72,P<0.05)、75.1%(K=0.59,P<0.05)、79.7%(K=0.62,P<0.05);HBV PreSl-Ag阳性患者血清ALT和AST升高率和水平均高于HBVPreSl-Ag阴性患者。结论HBV PreSl-Ag与HBV-DNA有较高的符合率,可作为反映HBV复制的指标。而且,HBV PreSl-Ag阳性患者血清ALT和AST水平高于阴性患者,表明HBVPreSl-Ag可反映肝功能状况。Objective To explore the clinical significance of HBV preS1 antigen in the diagnosis of viral replication. Methods The levels of HBV-DNA and HBV markers were measured by fluorescence quantitative PCR and enzyme linked immunosorbert assay （ELISA） respectively in 350 cases with HBV infection. The level of alanine aminotransferase was obtained by an automated biochemistry analyzer. Results No significant difference was found between the detectable rates of HBV DNA and HBV PreS1-Ag in patient with the same HBV markers. The correspondency rates of HBVPreS1-Ag and HBV-DNA,HBeAg and HBVPreS1-Ag,HBeAg and HBV DNA are 86.9% and 75.1% and 79.7% respectively . The average levels of ALT and AST in HBV PreS1-Ag-positive patients were higher than those in HBV PreS1-Ag negative ones. Conclusion HBV PreS1-Ag closely correlates with HBV DNA,and HBV PreS1-Ag is a reliable marker that can reflect the replication of HBV as well as liver function.

关 键 词：乙肝病毒前S1抗原 HBV DNA 丙氨酸氨基转移酶 门冬氨酸氨基转移酶 

分 类 号：R512.62[医药卫生—内科学] R373.21[医药卫生—临床医学]