基于纳米操纵的基因组测序技术与策略  

A sequencing strategy based on nanomanipulation

作　　者：吕鸣[1] 李雪玲[1] 吕军鸿[2] 邵志峰 胡钧[1] 

机构地区：[1]上海交通大学生命科学技术学院纳米生物学实验室 [2]中国科学院上海应用物理研究所 [3]Dept of Mol Physiol & Biol Phys,University of Virginia Medical School,Charlottesville,VA 22908-0736,USA

出　　处：《电子显微学报》2007年第5期488-493,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.10335070;No.10604061;No.10674147).~~

摘　　要：人类基因组计划所遗留下来的未能解决的序列(如人类基因组缺口,gaps)以及越来越多的测序需求,都迫切需要发展新一代的测序技术与策略。我们实验室长期从事基于原子力显微镜(AFM)对单个DNA分子进行纳米操纵方面的工作,在此基础上,最近提出并原理演示完成了一种单分子有序化测序策略。这种有序化测序策略与现有测序技术以及新兴高通量单分子测序技术的结合,有望解决基因组测序中的许多困难。　The increasing requirement for sequencing large amount of genomes calls for cheaper,faster,and more sensitive sequencing technology to overcome the limitations in current sequencing methods.We have devoted in developing new methods to manipulate single DNA molecules with atomic force microscope（AFM）at high spatial resolution.Based on AFM nanomanipulation,we have proposed a novel strategy for ordered single-molecule sequencing recently.In this paper,we reviewed our latest progresses and discussed the possibility of combination between our sequencing strategy and others,including conventional sequencing methods and the emerging ultra-high throughput single-molecule sequencing techniques.

关 键 词：有序化测序 单分子测序 测序策略 纳米操纵 原子力显微镜 

分 类 号：Q75[生物学—分子生物学]