hMSCs成骨诱导前后分泌免疫相关细胞因子的实验研究  

Experimental Study on Secretion of Immune-related Cellular Factors in DOC and hMSCs

作　　者：杨东明[1] 吴雪晖[1] 王蒙[1] 罗飞[1] 许建中[1] 

机构地区：[1]第三军医大学附属西南医院骨科全军矫形外科中心,重庆400038

出　　处：《现代生物医学进展》2007年第10期1451-1453,共3页Progress in Modern Biomedicine

基　　金：国家高技术研究发展资助重大攻关课题("863"计划)(2006-AA205020)

摘　　要：目的:研究hMSCs、DOC(osteogenic cells differentiated from mesenchymal stem cells,成骨诱导后骨髓间充质干细胞)分泌免疫相关细胞因子的变化,从细胞因子角度探讨hMSCs、DOC形成免疫抑制微环境的可能机理。方法:hMSCs、DOC(成骨诱导18天),采用ELISA技术,分别检测IL-2、IL-4、IL-10和TGF-β1分泌水平的变化。结果:在MSCs、DOC中IL-2、IL-4h低表达或忽略表达,IL-10中度表达,TGF-β1高表达。对IL-2、IL-4、IL-10,hMSCs、DOC的表达水平有统计学差异(P<0.05)。结论:在体外实验中,hMSCs、DOC可能形成免疫抑制微环境并通过其维持免疫调节功能。Objective： To investigate the variation of immune-related cellular factors in order to explain the possible mechanism of the immunosuppressive microenvironment of DOC and hMSCs. Methods： Expressions of IL-2, IL-4, IL-10 and TGF-β1 in DOC （diferentiated after 18 days） and undiferentiated hMSCs were detected through ELISA, Results： The cellular factors IL-2 and IL-4 were low-expressed, IL-10 was expressed at a middle level and TGF-β1 was expressed at a high level in DOC and hMSCs, Conclusion： It is suggested that DOC and hMSCs created and sustained an immunosuppressive microenvironment in vitro through their immune modulating functions.

关 键 词：HMSCS DOC ELISA 细胞因子 

分 类 号：Q78[生物学—分子生物学] Q813