富硒姬松茸提取物-硒蛋白多糖处理小鼠血清对K_(562)细胞凋亡的影响及机制  

An investigation of antitumor activity of mice serum treated with selenium-protein polysaccharide

作　　者：陈彻[1] 郝军[1] 楚惠媛[1] 刘永琦[1] 张迎梅[2] 

机构地区：[1]甘肃中医学院,兰州730000 [2]兰州大学生命科学院,兰州730000

出　　处：《中国新药杂志》2007年第17期1361-1364,共4页Chinese Journal of New Drugs

基　　金：甘肃省教育厅自然科学基金(J02-04);国家自然科学基金(30470320)

摘　　要：目的:探讨富硒姬松茸提取物-硒蛋白多糖(SPP)处理的小鼠血清在体外对K562细胞凋亡的影响及机制。方法:采用血清药理学法制备SPP处理血清,MTT法观察SPP处理的小鼠血清(终浓度10%)对K562细胞增殖的影响;从形态学、DNA电泳技术观察凋亡发生,比色法检测凋亡相关基因Caspase-3的表达。结果:SPP处理的小鼠血清可显著抑制白血病K562细胞生长,且呈时间剂量依赖性。形态学和DNA电泳结果均证实有凋亡发生。与空白血清组比较,Caspase-3基因上调明显。SPP处理的荷瘤小鼠血清抗肿瘤效果明显优于正常小鼠的处理血清。结论:SPP处理的荷瘤小鼠血清可促K562细胞凋亡,其机制可能与上调Caspase-3基因有关。Objective： To explore the effect and mechanism of serum treated with selenium-protein polysaccharide（SPP） , extracted from rich-selenium Agaricus blazei, inducing apoptosis of K562 ceils in vitro and compare the difference of antitumor activity of serum treated with SPP between normal and pathological mice. Methods： According to serum pharmacology, serum treated with SPP was prepared. Growth inhibition rate of K562 cells was detected by MTY method. Apoptotic phenomenon was observed under a fluorescent microscope and through DNA electrophoresis, respectively. Caspase-3 enzymatic activity was measured by colorimetry. Results：Serum treated with SPP（ 10% ）could significantly inhibit the growth of K562 ceils. There was a markedly positive correlation between drug dose （50,100 mg·kg^-1） , time（24 -48 h）and inhibitory rate. Apoptotic phenomenon was showed by morphology and DNA electrophoresis. Compared with control group, Caspase-3 gene was significantly up-regulated. Compared with the serum treated with SPP of nonbearing tumor group, that of bearing tumor group has much more potent effect on antitumor. Conclusion： Serum treated with SPP could induce apoptosis of K562 ceils. Its mechanism may be related to up-regulation of Caspase-3.

关 键 词：富硒姬松茸 硒蛋白多糖 血清药理学 凋亡 CASPASE-3基因 

分 类 号：R285.5[医药卫生—中药学] R286.91[医药卫生—中医学]