RP-HPLC法测定乙烷硒啉分散片中药物的含量  

A RP-HPLC method for determination of ethaselen dispersible tablets

作　　者：王存琴[1] 刘昕[2] 武凤兰[2] 

机构地区：[1]甘肃中医学院,兰州730000 [2]北京大学药学院,北京100083

出　　处：《中国新药杂志》2007年第17期1388-1390,共3页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30472036)

摘　　要：目的:建立乙烷硒啉分散片含量测定方法。方法:采用反相高效液相色谱法,使用BDS HYPERSILC18柱(250mm×4.6mm,5μm),检测波长为320nm,柱温为室温,流动相为0.01%磷酸-甲醇(60∶40),流速1.0mL.min-1。结果:溶剂与辅料对分散片中药物的含量测定无干扰,乙烷硒啉在10～400μg.mL-1范围内呈良好的线性关系(r=0.9995),精密度RSD为0.39%;日内精密度为0.57%;平均回收率为101.2%(n=9)。结论:该方法专属性强,操作方便,结果准确,重现性好,可用于乙烷硒啉分散片中药物的含量测定。Objective：To establish a RP-HPLC method for assaying ethaselen dispersible tablets. Methods：The chromatographic separation was performed on a BDS HYPERSIL C18 column （250 mm×4.6 mm, 5μm） at room temperature. The mobile phase consisted of CH3OH and 0. 01% H3PO4 （40： 60）. Flow rate was 1.0 mL·min^-1, with detection at 320 nm. Results：Calibration curve for eth aselen ranged from 10 to 400μg-mL^-1 （r =0. 999 5 ）. The average recovery was 101.2% （ n =9）. Conclusion ：A simple, accurate, and specific RP-HPLC method for assaying ethaselen dispersible tablets is established.

关 键 词：乙烷硒啉 分散片 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R979.1