高效液相色谱法测定工作场所空气中甲基丙烯酸的研究  

Determination of methacrylic acid in air of workplace by high performance liquid chromatography

作　　者：蒲国强[1] 范衍琼[1] 林小葵[2] 李玉萍[2] 冯婉丽[2] 

机构地区：[1]广东省江门市职业病防治所,广东江门529000 [2]广东省江门市新会区疾病预防控制中心,广东江门529100

出　　处：《中国卫生检验杂志》2007年第9期1574-1576,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立工作场所空气中甲基丙烯酸的溶剂解吸高效液相色谱测定方法。方法：以硅胶管采集空气中甲基丙烯酸，用甲醇解吸后高效液相色谱测定。结果：甲基丙烯酸浓度在0．24—300μg／ml（以采集3L空气样品计，相当于空气中甲基丙烯酸的浓度在0．24～300mg／m^3）线性关系良好，回归方程Y=74359644．5X＋96317．1，r=0．9993（n=6），检出限为0．24μg／ml；方法精密度试验，相对标准偏差在0．7％～3．6％之间；平均解吸效率98．4％；200mg硅胶管穿透容量（BTV）〉14．3mg；200mg硅胶吸附420μg甲基丙烯酸，密闭放置1周，损失率〈10％；采样效率达100％。结论：本方法适用于工作场所空气中甲基丙烯酸的浓度测定。Objectlve：To establish a HPLC analytical method for methacrylic acid in the air of workplace. Methods：Silica gel tube adsorption and solvent desorption were used for sampling of methacrylic acid and analysis was performed by HPLC. Results： The relative standard deviation in the range of 0. 24-300 μg/ml was 4. 7%-7.9%. Regression formula was Y = 74359644. 5X ＋ 96317. 1 ,r =0. 9993 （n =6）. Detection limit was 0. 24μg/ml （injection for 20 μl）. The average desorption efficiency was 98.4%. Breakthrough volume was 〉 14. 3 rag. In a tube containing 200 mg silica gel ,420 μg of methacrylic acid can be adsorbed. Keeping this tube tightly closed until a week,the methacrylic acid can still remain stable with a loss rate 〈 10%. The sampling efficiency was 100%. Condusion：The method is compatible with concentration detection of methacrylic acid in the air of workplace.

关 键 词：甲基丙烯酸 工作场所 硅胶管 溶剂解吸 高效液相色谱 

分 类 号：O657.72[理学—分析化学]