RSV毛细支气管炎RANTES MIP-1αIL-8水平变化及相关性研究  

Change of RANTES.MIP-1α and IL-8 levels in RSV bronchiolitis and their correlation

作　　者：董琳[1] 陈小芳[1] 周晓聪[1] 林剑[1] 李锦燕[1] 张伟[1] 

机构地区：[1]温州医学院附属育英儿童医院呼吸科,325027

出　　处：《浙江临床医学》2007年第10期1307-1308,共2页Zhejiang Clinical Medical Journal

基　　金：温州市跨世纪"551人才工程"资助项目

摘　　要：目的探讨呼吸道合胞病毒（RSV）毛细支气管炎（简称RSV毛支）RANTES、MIP-1α、IL-8的动态变化及相关性。方法RSV毛支组26例为住院的RSV毛细支气管炎患儿，年龄1．5-11．0个月；对照组为14例择期手术患儿，年龄5．4～15．1个月。应用ELISA法分别检测RSV毛支组急性期和恢复期鼻咽分泌物RANTES、MIP-1α、IL-8浓度。结果对照组鼻咽分泌物未测到RANTES和MIP-1α，测得低水平的IL-8；RSV毛细支气管炎患儿急性期RANTES、MIP-1α、IL-8明显高于对照组；恢复期RANTES、IL-8下降，但仍高于对照组；MIP-1α恢复期和急性期差异无显著性（P〉0．05），但高于对照组（P〈0．001）。RANTES与MIP-1α、IL-8及MIP-1α与IL-8间有正相关关系。结论RSV毛细支气管炎患儿RANTES、MIP-1α、IL-8明显升高，提示趋化因子可能在RSV感染的气道炎症中起重要作用。Objectives To explore the dynamic change of RANTES. MIP-1α and IL - 8 in children with respiratory syncytial virus （RSV） bronchiolitis and their correlation . Methods Twenty - six children aged 1.5 - 11.0 （4.7± 2.6）month hospitalized with bronchiolitis were enrolled as patient group. RSV infection diagnosis was based on direct immunofluorescence assay in NPS. Forteen children aged 5.4 - 15.1 month undergoing surgical operation were served as control subjects. NPS samples were collected from patients at acute stage and recovery stage respectively. All samples were analyzed for RANTES. MIP-1α and IL - 8 levels with ELISA. Results RANTES and MIP-1αconcentrations were not detected in NPS samples from control patients. Only low levels of IL - 8 were detected. Levels of RANTES. MIP-1α and IL - 8 were significantly greater in samples obtained from patients at acute stage than those of control children. Levels of RANTES and IL- 8 decreased significantly in patients at recovery stage and were greater than those in controls. Levels of MIP-1α in patients at recovery stage had no difference compared with those at acute stage, but were still higher than those in controls. Moreover, RANTES levels positively correlated both with MIP-1α and IL - 8 levels; Positive correlation between MIP-1α and IL - 8 levels was also apparent. Conclusion NPS concentrations of RANTES.MIP-1α and IL- 8 in children with RSV bronchiolitis increase significantly, which suggests an important role for chemokines in the airway inflammation induced by RSV.

关 键 词：趋化因子 鼻咽分泌物 毛细支气管炎 呼吸道合胞病毒 

分 类 号：R725.6[医药卫生—儿科]