利用转录增强技术检测草莓斑驳病毒  

Detection of Strawberry mottle virus by transcriptional enhancement techniques

作　　者：杨洪一[1] 代红艳[2] 李贺[2] 李丽丽[2] 张志宏[2] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]沈阳农业大学园艺学院,沈阳110161

出　　处：《植物保护学报》2007年第4期369-372,共4页Journal of Plant Protection

基　　金：国家自然科学基金项目(30200187)

摘　　要：为了提高检测草莓斑驳病毒(Strawberry mottle virus,SMoV)的灵敏度和稳定性,探索转录增强技术检测SMoV。在SMoV基因组3′端非编码区的保守区域设计引物,利用带有T7启动子序列的引物进行反转录聚合酶链式反应(RT-PCR),再利用T7RNA聚合酶对PCR产物进行体外转录,最后直接通过电泳检测转录产物。结果表明,利用转录增强技术可稳定检测SMoV,灵敏度高于常规RT-PCR;T7启动子序列加在正义引物或反义引物上都可以有效检测。Strawberries production was damaged severely by Strawberry mottle virus （SMoV）. In order to improve the stability and sensitivity of detection of SMoV, we attempted to detect SMoV by transcriptional enhancement techniques. The primers were designed in the conservative non-coding region of the 3′ end of SMoV genome. The primers with T7 promoter sequence were applied in reverse transcription-polymerase chain reaction （RT-PCR）. The transcription in vitro for PCR products was brought into effect by 37 RNA polymerase, and then the products were separated by electrophoresis in agarose gels. The results showed that SMoV could be detected steadily by transcriptional enhancement techniques. It was more sensitive than standard RT-PCR. Both were good when the 37 promoter sequence was added sense primer or antisense primer.

关 键 词：转录增强 草莓斑驳病毒 检测 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]