雌激素对兔视网膜缺血再灌注损伤中谷氨酸浓度的影响  

Effect of β-estradiol on glutamate concentration in retina injured by ischemic reperfusion

作　　者：朱远军[1] 金敏[1] 高宗银[1] 杨为中[1] 胡燕飞[1] 郝绮红[1] 

机构地区：[1]广州医学院附属广州市第一人民医院眼科,510180

出　　处：《中华眼底病杂志》2007年第5期321-323,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：广东省科技计划项目（2002C30903）

摘　　要：目的探讨雌激素对视网膜缺血再灌注（RIR）兔眼模型视网膜组织中游离谷氨酸浓度的影响。方法新西兰白兔20只随机分为对照组和实验组,每组各10只兔。各组动物在行双眼闪光视网膜电图（FERG）后,右眼应用前房灌注加压法使前房内压力升高至120mmHg（1mmHg=0.133kPa）,维持60min后再灌注,建立RIR模型;左眼为空白对照眼。实验组在前房灌注前2h皮下注射β-雌二醇（0.1mg/kg）,对照组给予等量生理盐水皮下注射。再灌注即时及4、8、24h后分别记录各组右眼FERG,与缺血前FERG比较,观察a、b波振幅变化。最后一次ERG检测后,立即摘除所有兔双眼眼球,采集视网膜组织,研磨及蛋白沉淀后,使用日立L-8800氨基酸自动分析仪检测游离氨基酸浓度。结果两组兔右眼在结束造模后即时FERG图形均呈直线;对照组与实验组再灌注各时段FERGa波振幅差异无统计学意义（t=1.357,0.798,0.835;P〉0.05）;实验组各时段b波振幅明显高于对照组,差异有统计学意义（t=4.447,2.188,3.106;P〈0.05）。兔视网膜组织中游离谷氨酸浓度分别为：对照组右眼（0.265±0.014）g/L,左眼（0.207±0.013）g/L;实验组右眼（0.231±0.007）g/L,左眼（0.203±0.014）g/L,两组差异有统计学意义（F=50.807,P=0.000）;对照组双眼间、实验组双眼间以及右眼两组间的差异有统计学意义（P=0.000）;两组左眼间差异无统计学意义（P=0.505）。结论雌激素可以阻止RIR眼视网膜组织中游离谷氨酸浓度升高,从而保护视网膜组织。Objective To observe the effect of β-estradiol on glutamate concentration in rabbits＇ retinae injured by ischemic reperfusion. Methods Twenty rabbits ware randomly divided into two groups, the control group and the treatment group, with 10 rabbits in each group. Before examined by binocular flash electroretinography （FERG）, retinal ischemic reperfusion （RIR） model was induced in the right eyes of all the rabbits by increasing intraocular pressure to 120 mm Fig for 60 minutes; the left eyes were as the control eyes. The rabbits were hypodermically injected with β-estradiol （0. 1 mg/kg） in treatment group and with physiological saline in the control group 2 hours before ischemia. The results of FERG of the right eyes in both of the 2 groups 0, 4, 8, and 24 hours after reperfusion were record respectively and were compared with the results of FERG before reperfusion. The retina tissue was collected after the last time of FERG. The concentration of glutamate was detected by Hitachi L-8800 amino acid analyzer. Results In the right eyes in both of the 2 groups, the result of FERG showed a beeline just after reperfusion. There was no significant difference of a wave amplitude between the 2 groups （t=1.357, 0.798, 0.835; P〉0.05）; the b wave amplitudes in experimental group were much higher than those in the control group （t = 4.447, 2. 188, 3. 106; P〈0.01）. The concentration of glutamate in retina was （0. 265±0. 014） g/L in the right eyes and （0. 207±0. 013） g/L in the left eyes in the control group, and （0. 231±0. 007） g/L in the right eyes and （0. 203±0. 014） g/L in the left eyes in the treatment group ; the difference between the 2 groups was significant （F= 50. 807, P= 0. 000）. There was statistical difference between right and left eyes both in the 2 groups and the significant difference of the right eyes between the two groups was also found （P=0. 000）; there was no statistical difference of the left eyes between the 2 groups （P=0. 505）. Conclusion β-estr

关 键 词：雌激素类/生理学 再灌注损伤 谷氨酸 视网膜电描记术 动物实验 

分 类 号：R686[医药卫生—骨科学]