光相干断层扫描连续监测大鼠慢性高眼压模型视盘神经纤维厚度变化  

Changes of thickness of retinal nerve fiber layer of optic disc in rats with chronic glaucoma continuously detected by optic coherence tomography

作　　者：刘东敬[1] 陈晓明[1] 孟丹[1] 鲍捷[1] 盘如刚[1] 李妮[1] 刘娅利[1] 李矛[1] 

机构地区：[1]四川大学华西医院眼科,成都610041

出　　处：《中华眼底病杂志》2007年第5期352-354,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的用光相干断层扫描（OCT）连续观测大鼠慢性高眼压模型视盘神经纤维层（RNFL）厚度的变化。方法选用Wistar大鼠48只,随机分为3组,每组16只鼠32只眼,右眼为激光光凝眼,左眼为对照眼。用波长为532nm氩激光在全麻下光凝右眼小梁网,引起眼压慢性、中等程度升高并观测眼压变化。眼压升高后第3、6、9周时用OCT做视盘线性扫描,计算机自动测量视盘RNFL厚度,然后处死大鼠,将每组8只大鼠右眼做光学切片行组织学测量RNFL厚度,将另外8只大鼠右眼做全视网膜铺片甲苯胺蓝染色,记数视网膜神经元细胞密度,将结果进行比较分析。结果激光光凝后大鼠眼压缓慢、中等程度升高,在第3、6、9周时光凝眼眼压分别比对照眼眼压为显著升高,差异有统计学意义（P〈0.001）。OCT检查结果显示在3、6、9周时大鼠光凝眼视盘RNFL厚度分别小于对照眼,差异有统计学意义（P〈0.05）。处死大鼠后组织学测量RNFL厚度,在3、6、9周时,光凝眼为（64.38±6.54）、（51.47±6.4）、（42.10±6.10）μm,对照眼厚度为（76.23±6.78）、（78.64±6.15）、（77.64±6.63）μm。将两种方法测得RNFL厚度值进行回归分析,两者变化趋势一致,相关系数（R=0.932,P〈0.001）。全视网膜铺片甲胺蓝染色结果显示两组视网膜神经元细胞（RGC）密度值差异有统计学意义（P〈0.05）。结论激光光凝大鼠小梁可以成功建立大鼠慢性高眼压模型;OCT对大鼠慢性高眼压模型视盘RNFL厚度的测量与在光学显微镜下的测量值变化趋势一致,相关性好;OCT可以连续活体监测大鼠慢性高眼压模型视盘神经纤维厚度变化,从而了解大鼠青光眼视神经病变的进展。Objective To observe the changes of the thickness of retinal nerve fiber layer （RNFL） of optic disc in rats with chronic glaucoma continuously detected by optic coherence tomography （OCT）. Methods A total of 48 Wistar rats （24 males and 24 females） were randomly divided into 3 groups with 16 rats （32 eyes） in each group. The right eyes were the photocoagulation eyes and the left ones were as the control. Laser photocoagulation with the wavelength of 532 nm was performed on the trabecular network of the right eyes to induce the chronic middle-level ocular hypertension. The changes of the intraocular pressure （IOP） were observed. Optic-disc linear scanning of OCT was performed 3, 6, and 9 weeks after IOP increased, and the thickness of RNFL of optic disc was detected by the computer. Eight rats in each group were killed and retinal histology slices were used to detect the thickness of RNFL. The flatmounts of retina from the right eyes of the other 8 rats in each group were stained by 1% toluidine blue. The density of retinal ganglion cells （RGC） was calculated and the results were compared and analyzed. Results IOP of the rats increased chronically and moderately after photocoagulation. IOP of the experimental eye 3, 6, and 9 weeks after photocoagulation was obviously higher than which of the control eyes, respectively （P〈0. 001）. The results of OCT showed that the thickness of the RNFL of the experimental eyes was （67.39±5.91） μm, （53.42±5.64） μm,and （44.35±5.76） μm 3, 6, and 9 weeks after photocoagulation, and the corresponding thickness in the control eyes was （80. 32 ± 5.87）, （79.69 ± 5.69）, and （80. 78± 5.84）μm, respectively. The thickness of the retinal fiber layer detected by histological method was （64.38±6.54）, （51.47±6.4）, and （42. 10±6. 10）μm in the experimental eyes 3, 6, and 9weeks after photocoagulation, and （76.23±6.78）, （78.64±6.15）, and （77.64±6.63） μm in the control eyes. Regression analysis of the thi

关 键 词：高眼压 视盘 神经纤维/病理学 体层摄影术 光学相干 动物实验 

分 类 号：R686[医药卫生—骨科学]