嗜麦芽寡养单胞菌中Ⅱ类整合酶基因的发现及意义  

Detection and Significance of Class Ⅱ Integrons in Clinically Isolated Strains of Stenotrophomonas maltophilia

作　　者：邓笑伟[1] 刘长庭[1] 李天智[1] 王俊锋[1] 粱立武[2] 黄燕萍[2] 莫晨[2] 

机构地区：[1]解放军总医院,北京100853 [2]武警总医院,北京100039

出　　处：《中华医院感染学杂志》2007年第9期1061-1063,共3页Chinese Journal of Nosocomiology

基　　金：北京市科委重大培育专项(Z0005190041891)

摘　　要：目的检测Ⅰ、Ⅱ、Ⅲ类整合子在嗜麦芽寡养单胞菌临床分离株中存在情况,分析整合子对细菌耐药性的影响。方法应用多重PCR方法筛选嗜麦芽寡养单胞菌整合酶基因。结果Ⅰ类整合子的检出率为13.4%,其中3株菌同时带有Ⅰ类和Ⅱ类整合子,Ⅱ类整合子的阳性率为3.6%,未检测到Ⅲ类整合子。结论嗜麦芽寡养单胞菌中主要分布Ⅰ类整合子,首次发现嗜麦芽寡养单胞菌中含有Ⅱ类整合子,Ⅱ类整合酶基因可能增加细菌多药耐药的发生。OBJECTIVE To investigate the distribution and characterization of the classes Ⅰ , Ⅱand Ⅲ integrons on Stenotrophomonas maltophilia and clarify their influence on the bacterial drug-resistance. METHODS A multi- PCR assay using specific primers of int1, int2 and int3 was constructed to screen classesⅠ , Ⅱand Ⅲ integrons. RESULTS Class Ⅰ integron was detected in 13. 4% of clinical isolates, 3 isolates harbored among classⅡ integrons. There was not been reported in abroad. CONCLUSIONS Classes Ⅰand Ⅱintegrons could play an important role in causing the antibiotic multidrug resistance.

关 键 词：嗜麦芽寡养单胞菌 整合子 多重PCR 

分 类 号：R372[医药卫生—病原生物学]