周期性牵张对肺泡Ⅱ型上皮细胞IL-8表达的影响  

Effect of cyclic mechanical stretch on IL-8 expression in type Ⅱ alveolar epithelial cells

作　　者：宁巧明[1] 王祥瑞[1] 

机构地区：[1]上海交通大学医学院附属仁济医院麻醉科,200127

出　　处：《中华麻醉学杂志》2007年第8期728-731,共4页Chinese Journal of Anesthesiology

基　　金：上海市卫生局基金资助项目（03-77-20）

摘　　要：目的研究周期性牵张肺泡Ⅱ型上皮细胞株A549细胞对炎性介质白细胞介素-8（IL8）产生和释放的影响。方法对融合的肺泡Ⅱ型上皮细胞株A549细胞施加周期性机械牵张应力。实验一：加载频率0．5Hz，加载时间4h，加载应力分别为5％、15％、30％；实验二：加载应力为15％，加载频率0．5Hz，加载时间分别为1h、2h、4h、8h；实验三：加载应力为15％，加载时间4h，加载频率分别为0．2Hz、0．5Hz、1．0Hz。每个实验均设立空白对照即不给予机械应力。用实时荧光定量PCR法测定IL-8 mRNA的表达，用ELISA法测定IL-8蛋白的浓度。结果随着加载应力的增加和加载时间的延长，IL-8蛋白浓度增加、IL-8 mRNA表达上调（P〈0．05）；施加不同加载频率后，IL-8蛋白浓度增加、IL-8 mRNA表达上调（P〈0．05）；不同加载频率间IL-8蛋白浓度和IL-8 mRNA表达比较差异无统计学意义（P〉0．05）。结论肺泡Ⅱ型上皮细胞IL-8的产生和释放与周期性的机械牵张应力呈强度和时间依赖性，而加载频率对其无影响。Objective To investigate the effect of cyclic mechanical stretch of type Ⅱ alveolar epithelial cells on the production and release of IL-8. Methods The type Ⅱ alveolar epithelial cell line A549 cells were exposed to cyclic mechanical stretch. The experiment was performed in 3 parts. In part Ⅰ A549 cells were exposed to different strain （5%, 15%, 30% ） at frequency of 0.5 Hz for 4 hours. In part Ⅱ A549 cells were stretched for different length of time （1 h, 2 h, 4 h, 8 h） at strain 15%, and 0.5 Hz. In part m A549 cells were stretched with different frequencies （0.2 Hz, 0.5 Hz, 1.0 Hz） at strain 15% for 4 h. The expression of IL-8 mRNA in the cells and protein in supernatant was assessed using quantitative polymerase chain reaction and enzyme linked immunosorbent assay.Results A549 cells subjected to cyclic stretch produced and released IL-8 protein in a time and intensity dependent manner. The frequency of cyclic stretch had no effect on the expression of IL-8 mRNA and protein. Conclusion Cyclic mechanical stretch of type Ⅱ epithelial cells can induce production and release of IL-8 in a time and intensity dependent manner. The frequency of cyclic stretch does not affect IL-8 expression.

关 键 词：应力 物理 白细胞介素8 肺泡 上皮细胞 

分 类 号：R563[医药卫生—呼吸系统]