正常人外周血中CD8^+CD25^+T细胞亚群的数量及其细胞因子表达的初步研究  

The preliminary study on the numbers and cytokine expression of CD8^+CD25^+T cells from human peripheral blood

作　　者：王卫文[1] 张戎[1] 杨晓帆[1] 夏永祥[2] 李有武[2] 王慧娟[1] 季晓辉[1] 

机构地区：[1]南京医科大学微生物学与免疫学系,江苏南京210029 [2]南京医科大学附属南京第一医院检验科,江苏南京210006

出　　处：《南京医科大学学报（自然科学版）》2007年第10期1092-1097,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金资助(NY02069)

摘　　要：目的:研究正常人外周血CD8+CD25+T细胞亚群的数量及其表达IFN-γ、IL-10和TGF-β的细胞数量情况,以期得到一组该亚群细胞正常数值范围,为研究CD8+CD25+T细胞亚群在自身免疫性疾病、过敏性疾病和器官移植中的变化及其临床意义提供参考。方法:分离正常人外周血单个核细胞,利用三色流式细胞术分别检测CD3+T细胞中CD8+CD25+、CD8+CD25-、CD8-CD25+和CD8-CD25-亚群的比例,并计算其细胞数量;并对经过或未经过离子霉素(ionomycin)、佛波醇乙酯(PMA)联合刺激5h的CD8+CD25+T细胞检测表达IFN-γ、IL-10及TGF-β的细胞数量情况。结果:正常人外周血CD3+T细胞中CD8+CD25+细胞亚群约为2.52%,数量达37.09×106/L,经离子霉素、佛波醇乙酯联合刺激培养5h后其比例、数量均略有降低;CD8+CD25+T细胞表面TGF-β阳性率达到93.8%,刺激对其表达无明显影响;但刺激后IFN-γ+T细胞明显增多,而IL-10+的T细胞未见明显升高。结论:正常人外周血CD3+T细胞中存在少量的CD8+CD25+细胞亚群,该细胞表面稳定地高表达TGF-β,可对刺激呈现IFN-γ反应,但不对刺激产生增殖反应。Objective：In order to know the clinical significance of the CD8^＋CD25^＋T cell in autoimmune disease,hypersensitivity disease and organ transplantation, the normal limits of this T cell subset was obtained by studying the number ofCD8^＋CD25^＋T cell and its productions of IFN-γ,IL-10 and TGF-β in the normal peripheral blood. Methods：Mononuclear cells were isolated from healthy donors. The percentages and absolute numbers of CD8^＋CD25^＋,CD8^＋CD25^-,CD8^-CD25^＋ and CD8^-CD25^- from CD^3＋ T cells were detected by flow cytometry. In addition, stimulating with/without ionomycin and PMA 5 hours later,the expressions of IFN-γ, IL- 10 and TGF-β in the CD8^＋CD25^＋ T cells was measured. Results：The percentage of CD8^＋CD25^＋ subgroup in CD^3＋ T cells from normal human peripheral blood was 2.52% ,the absolute number was 37.09×10^6/L. The percentage and absolute number decreased after stimulated with ionomycin and PMA 5 hours. The expression of TGF-β on the surface of CD8^＋CD25^＋ T cells up to 93.8% ,stimulation seems not obviously affected its expression but IFN-γ T cells increased obviously,however,IL-10^＋ T cells didn＇t obviously up-regulal. Conclusion：There were a few CD8^＋CD25^＋ T cells in CD3^＋ T cells from normal peripheral blood. They expressed highly TGF-β in the cell surface,and could have IFN-γ reaction but no proliferation after stimulation.

关 键 词：调节性T细胞 CD8^+CD25^+细胞亚群 TGF-β IL-10 IFN-Γ 

分 类 号：R392.12[医药卫生—免疫学]