急性冠状动脉综合征患者淋巴细胞电压依赖性钾通道的变化  

T-lymphocyte voltage dependent K^+ channel is upregulated in patients with acute coronary syndrome

作　　者：郭丽芬[1] 张存泰[1] 吴杰[1] 刘念[1] 孙莉萍[1] 刘俊[1] 马金[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心内科,武汉430030

出　　处：《中华心血管病杂志》2007年第9期818-821,共4页Chinese Journal of Cardiology

摘　　要：目的用全细胞膜片钳技术和 Western 印迹法,探讨急性冠状动脉综合征(ACS)患者外周血淋巴细胞电压依赖性钾通道(Kv)电流及 Kv1.3蛋白表达的变化。方法收集12例 ACS 患者和10例健康志愿者外周血淋巴细胞,采用膜片钳全细胞电流记录方法,记录淋巴细胞膜 Kv 的电流密度。用 Western 印迹法检测外周血淋巴细胞 Kv1.3蛋白的表达。结果 ACS 患者淋巴细胞 Kv 的电流密度为(269±94)pA/pF,明显高于正常人[(191±64)pA/pF,P<0.01]。ACS 组淋巴细胞膜电容为(2.3±0.7)pF,对照组为(2.2±0.5)pF,两组间差异无统计学意义。Western 印迹结果显示 ACS 患者淋巴细胞 Kv1.3通道表达高于正常人(P<0.01)。结论 ACS 患者淋巴细胞 Kv 表达明显增多,说明 Kv 在调节 ACS 患者淋巴细胞的激活中起着关键性作用。Objective To determine current density of voltage-gated potassium channels and Kvl. 3 express in T-lymphocyte derived from patients with acute coronary syndrome （ACS）. Methods Peripheral blood mononuclear ceils were collected from 12 patients with ACS and 10 control donors. Whole-cell patch clamp technique was used to record the outward K^± currents （IK） and western blots technique was used to detect the express of Kv1. 3 protein in lymphocyte. Results （ 1 ） The current density of voltage-gated potassium channel was significantly higher in ACS patients [ （269 ± 94）pA/pF] than in controls [ （191 ± 64） pA/pF, P 〈0.01 ] while membrane capacitance was similar between the two groups. （2） Kvl. 3 protein expression was also significantly increased in ACS patients than in controls （P 〈 0.01 ）. Conclusion The lymphocyte voltage-gated potassium channel is upregulated in patients with ACS suggesting a role of Kv activation in the pathophysiology of ACS.

关 键 词：冠状动脉疾病 淋巴细胞 钾通道 

分 类 号：R541.4[医药卫生—心血管疾病]