低分子质量硫酸葡聚糖对人血清灌注下小鼠离体心脏的保护作用  

Protective effects of low-molecular dextran sulfate on the isolated hearts in mice in ex vivo human serum perfusion model

作　　者：徐婧[1] 谢林[1] 陈刚[1] 陈松[1] 黄亚冰[1] 尹注增[1] 陈实[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所教育部/卫生部器官移植重点实验室,武汉430030

出　　处：《中华器官移植杂志》2007年第9期522-524,共3页Chinese Journal of Organ Transplantation

基　　金：国家高新技术发展研究计划("863"计划)基金(2003AA205009);高等学校博士学科专项基金(20040487077)

摘　　要：目的研究相对分子质量为5000的硫酸葡聚糖(DXS)对人血清灌注下小鼠离体心脏的保护作用及其可能机制。方法切取Balb/c小鼠的心脏,采用改良Langendorff灌注装置,以含人血清的灌注液进行心脏灌注,实验组1和实验组2的灌注液中加入DXS(终浓度分别为0.1 mg/ml和2.0mg/ml),以不含DXS的人血清灌注液为对照组,记录心脏搏动时间,对照组心脏停搏时,同时切取3个组的心脏组织,HE染色,观察组织学变化,免疫组化方法观察心脏组织中补体C3c、C5b-9和IgM、IgG的沉积情况。结果实验组1心脏搏动时间为(49±15)min,实验组2为(123±19)min,对照组为(17±11)min,两个实验组的心脏搏动时间均显著长于对照组(P<0.05)。HE染色见对照组心肌组织水肿明显,组织间隙变宽,而在灌注相同时间的实验组心脏中此改变不明显。免疫组化染色显示实验组2的心脏组织内C3c和C5b-9沉积较对照组明显减少。结论低分子质量的DXS对人血清灌注下的小鼠离体心脏具有保护作用,这可能与DXS抑制补体激活,从而抑制补体介导的异种超急性排斥反应有关。Objective To investigate the protective effects of low molecular weight dextran sulfate （DXS, molecular weight 5000） on the isolated hearts in mice in ex vivo human serum perfusion model and the possible mechanism. Methods The hearts of balbc mice were isolated and perfused with 10 pooled human serum of B type to which 0. 1 （experimental group 1） or 2 mg/ml DXS （experimental group 2） was added. The hearts perfused with 10 % pooled human serum alone served as control group. Immunoflurescence staining and immunohistochemistry were used to detect the deposition of complement C3c, C5b-9 and anti-human IgM, IgG. Results The mean survival time in con trol group, experimental group 1 and experimental group 2 was 17± 11, 49 ± 15 and 123 ± 19 min respectively. There was significant difference between the experimental groups and control group （P〈 0. 05）. The deposition of complement C3c and C5b-9 was significantly decreased in experimental group 2 as compared with that in control group. Conclusion DXS inhibits the complement activation and protects mouse heart function.

关 键 词：硫酸葡聚糖 血清 血液灌注 小鼠 人类 心室功能 左 

分 类 号：R654.2[医药卫生—外科学]