低温氧合血微流量持续灌注保存大鼠心脏的效果  

The effects of continuous oxygenated blood microperfusion on rat hearts preservation

作　　者：高宏[1] 臧旺福[2] 田海[1] 高伟 

机构地区：[1]哈尔滨医科大学附属第二医院心外科,150086 [2]上海交通大学医学院附属瑞金医院心胸外科 [3]黑龙江省兰西县人民医院外科

出　　处：《中华器官移植杂志》2007年第9期537-540,共4页Chinese Journal of Organ Transplantation

摘　　要：目的探讨低温氧合血微流量持续灌注保存大鼠心脏的效果。方法切取Wistar大鼠心脏,随机分为3组,实验组的心脏以4℃氧合血微流量(1 ml/h)持续灌注低温保存10 h;对照组的心脏以4℃St.Thomas液微流量持续灌注低温保存10 h;单纯冷保存组的心脏以4℃St.Thomas液单纯浸泡保存10 h。保存后的鼠心用Langendorff装置灌注,生物机能实验系统测定血流动力学指标;高效液相色谱仪测定心肌细胞的ATP含量;光镜和电镜观察心肌组织和线粒体的形态学改变。结果在再灌注30 min时,实验组的左心室收缩压(LVSP)为(38.25±3.84)mm Hg,左心室发展压(LVDP)为(32.54±4.01)mm Hg,左心室压力微分(±dp/dt)为(1080±123)mm Hg/s;对照组的LVSP为(34.48±4.68)mm Hg,LVDP为(19.27±4.63)mm Hg,±dp/dt为(935±196) mm Hg/s;单纯冷保存组的LVSP为(32.14±4.95)mm Hg,LVDP为(16.99±4.85)mm Hg,±dp/dt为(825±302)mm Hg/s,实验组的上述血流动力学指标优于对照组和单纯冷保存组(P<0.05)。实验组心肌细胞的ATP含量为(1.759±0.502)μmol/L,对照组的ATP为(1.453±0.573)μmol/L,单纯保存组的ATP为(1.059±0.463)μmol/L,实验组的ATP含量明显高于对照组和单纯冷保存组(P<0.05)。实验组的心肌组织和细胞超微结构的变化轻于对照组和单纯冷保存组。结论低温氧合血微流量持续灌注能改善大鼠心脏的保存效果。Objective To study the effects of continuous oxygenated blood microperfusion on rat hearts preservation. Methods Forty-five Wistar rats were randomly divided into 3 groups. The isolated rat hearts were continuously microperfused with oxygenated blood at 4 ℃ in experimental group, those in control group microperfused with St. Thomas solution and those in solution group were preserved with St. Thomas solution. After preservation the hemodynamic indexes of rat hearts were measured using Langendorff system, including the left ventricular end-diastolic pressure （LVEDP）, the left ventricular systolic pressure （LVSP）, the left ventricular developed pressure （LVDP） and the rate of the left ventricular pressure change （ ± dp/dt）, then the contents of adenosine triphosphate （ATP） were determined by high-performance liquid chromatography and myocardium ultrastructures were observed under an electric microscope. Results After 30 min perfusion of Langendorff system, the LVSP, LVDP and ± dp/dt were （38. 25±3.84） mm Hg, （32. 54± 4. 01） mm Hg and （1080 ± 123） mm Hg/s respectively in experimental group; （34. 48 ±4. 68） mm Hg, （ 19. 27 ± 4. 63） mm Hg and （935 ± 196） mm Hg/s respectively in control group; （32. 14 ± 4. 95） mm Hg, （16.99 ±4. 85） mm Hg and （825 ±302） mm Hg/s respectively in solution group. The hemodynamic indexes in experimental group were superior to those in control group and solution group （P〈0. 05）. The contents of ATP in experimental group, control group and solution group were （1. 759 ± 0. 502）, （1. 453 ± 0. 573） and （ 1. 059 ± 0. 463） μmol/L respectively. The contents of ATP in experimental group were significantly higher than those in control group and solution group （P〈0. 05）. The changes of myocardium ultrastructure in experimental group were milder than those in control group and solution group. Conclusion Continuous oxygenated blood microperfusion can improve the hypothermic preservation effects of rat hearts.

关 键 词：血液灌注 器官保存 细胞保护 心脏 大鼠 

分 类 号：R654.2[医药卫生—外科学]