[Ru(phen)_2dppz]^(2+)二聚及对DNA键合性质的影响  

Effects of the Dimerization of [Ru(Phen)_2dppz]^(2+) on the Calf Thymus Binding Properties

作　　者：聂晶[1] 韩美娇[1] 王科志[1] 

机构地区：[1]北京师范大学化学学院,北京100875

出　　处：《高等学校化学学报》2007年第10期1833-1835,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20371008;90401007);北京市自然科学基金(批准号:2052008;2072011);高等学校博士学科点专项科研基金(批准号:20060027002);北京师范大学本科生科学研究基金资助.

摘　　要：The dimerization of a well-known DNA light-switch [Ru(phen)2dppz]2+(phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2′,3′-c]phenazine), and its effect on calf thymus binding properties were studied by UV-Vis and emission spectroscopy in Tris-HCl buffered 50 mmol/L NaCl(pH=7.1). The results show that the complex has a strong tendency to dimerize with a dimerization constant of (1.8±0.3)×106 L/mol at the same order of magnitude as the DNA binding constant of (6.0±1.2)×106 L/mol for the Ru(Ⅱ) complex dimer, but one order of magnitude less than (4.9±1.5)×107 L/mol for the Ru(Ⅱ) complex monomer. This study has provided insight into a fundamental but important issue that should be taken into account for the DNA binding studies.The dimerization of a well-known DNA light-switch [ Ru（phen） 2dppz]^2 ＋（ phen = 1,10-phenanthroline, dppz = dipyrido [ 3,2-a ： 2＇, 3＇-c] phenazine）, and its effect on calf thymus binding properties were studied by UV-Vis and emission spectroscopy in Tris-HCl buffered 50 mmol/L NaCI（pH = 7. 1 ）. The results show that the complex has a strong tendency to dimerize with a dimerization constant of （1.8±0.3 ） × 10^6 L/ mol at the same order of magnitude as the DNA binding constant of （6.0 ± 1.2） × 10^6 L/mol for the Ru（ Ⅱ ） complex dimer, but one order of magnitude less than （4. 9 ±1.5 ） × 10^7 L/mol for the Ru（ Ⅱ ） complex monomer. This study has provided insight into a fundamental but important issue that should be taken into account for the DNA binding studies.

关 键 词：钌 邻菲啰啉 DNA 光开关 二聚 

分 类 号：O614[理学—无机化学] O641.3[理学—化学]