三甲氧基苄啶分子印迹整体柱的制备及色谱性能  

Preparation of Molecularly Imprinted Monolith for Trimethoprim Molecule and Its Chromatographic Property

作　　者：刘祥军[1] 刘吉众[1] 赵睿[1] 刘国诠[1] 陈义[1] 

机构地区：[1]北京分子科学国家实验室中国科学院化学研究所,北京100080

出　　处：《高等学校化学学报》2007年第10期1878-1880,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20575072;20435030)资助.

摘　　要：A monolithic molecularly imprinted polymer(monolithic MIP) for trimethoprim(TMP) was prepared by in situ polymerization method as the HPLC stationary phase. Methacrylic acid(MAA) and ethylene glycol dimethacrylate(EDMA) were used as functional monomer and cross-linker, respectively. The obtained MIP monoliths were characterized by high performance liquid chromatography and scanning electron microscope. The SEM analysis result clearly shows that many macropores and flow-through pores were embedded in the network skeleton of the TMP-imprinted monolith, which allowed the mobile phase to flow through the monolithic MIPs with a very low flow resistance. When the flow rate was high to 9.0 mL/min, the backpressure was only 11.1 MPa. Moreover, the imprinting factor was up to 10.3, which demonstrates that the obtained monolithic MIP showed a strong affinity and selectivity to the template molecule.A monolithic molecularly imprinted polymer （monolithic MIP） for trimethoprim （TMP） was prepared by in situ polymerization method as the HPLC stationary, phase. Methacrylic acid（MAA） and ethylene glycol dimethacrylate（EDMA） were used as functional monomer and cross-linker, respectively. The obtained MIP monoliths were characterized by high performance liquid chromatography and scanning electron microscope. The SEM analysis result clearly shows that many macropores and flow-through pores were embedded in the network skeleton of the TMP-imprinted monolith, which allowed the mobile phase to flow through the monolithic MIPs with a very low flow resistance. When the flow rate was high to 9.0 mL/min, the backpressure was only 11.1 MPa. Moreover, the imprinting factor was up to 10.3, which demonstrates that the obtained monolithic MIP showed a strong affinity and selectivity to the template molecule.

关 键 词：分子印迹聚合物 整体柱 三甲氧基苄啶 

分 类 号：O657.32[理学—分析化学]