反相高效液相色谱法同时测定人血浆中替米沙坦、氢氯噻嗪含量  

Simultaneous Determination of Telmisartan and Hydrochlorothiazide in Human Plasma by RP-HPLC

作　　者：周素琴[1] 李芳[1] 赵慧[1] 于香安[1] 

机构地区：[1]兰州大学第二医院药剂科,兰州730030

出　　处：《中国药学杂志》2007年第19期1496-1498,共3页Chinese Pharmaceutical Journal

摘　　要：目的建立可同时检测人血浆中替米沙坦、氢氯噻嗪含量的反相高效液相色谱方法。方法色谱条件：色谱柱为ZorbaxsoA Extend-C18（4.6mm×150mm，5μm）；流动相为乙腈-四丁基氢氧化铵磷酸盐缓冲液（24：76）；流速：1.0mL·min^-1；检测波长：230nm（替米沙坦），272nm（氢氯噻嗪）；柱温：20℃。结果本法替米沙坦的线性范围为3l.8～l591.2μg·L^-1，r=0．9994．方法回收率为98．68％，RSD=3．09％，最低检测质量浓度为20μg·L^-1，氢氯噻嗪的线性范围为30～240μg·L^-1，r=0．9991．方法回收率为100．32％，RSD=5．11％，最低检测质量浓度为20μg·L^-1结论本法可以同时测定血浆中替米沙坦和氢氯噻嗪的浓度，具有样品处理简单、方法稳定、有较好的实用性，易于开展药动学及药效学研究。OBJECTIVE To establish a method for simultaneous determination of telmisartan and hydrochlorothiazide in human plasma. METHODS The concentrations of telmisartan and HCTZ were analysed on a Zorbax 80A Extend -C18 （4.6 mm×150 mm,5 μm） column with a mobile phase consisting of tetrabutylammonium hydroxide phosphate buffer solution （ pH 2.26 ） -acetonitrile （76： 24 ） at a flow rate of 1 mL · min^-1, the wavelengthes were 230 and 272 nm, respectively. RESULTS The calibration curve of TMST was linear with the range of 31.8 - 1 591.2 μg · L^-1. The minimum eoncentration was 20 μg · L^-1. The calibration curve of hydrochlo- rothiazide was linear with the range of 30 - 240 μg · L^-1. The minimum concentration was 20 μg · L^-1. CONCLUSION It is a rapid, reproducible method. Clinical application demonstrated that this method was practical and it can be completely used in the study of pharmacokinetics and pharmacodynamics.

关 键 词：高效液相色谱法 替米沙坦 氢氯噻嗪 血浆浓度 

分 类 号：R917[医药卫生—药物分析学]