反相高效液相色谱法同时测定人血浆中替米沙坦、氢氯噻嗪含量  被引量:7

Simultaneous Determination of Telmisartan and Hydrochlorothiazide in Human Plasma by RP-HPLC

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作  者:周素琴[1] 李芳[1] 赵慧[1] 于香安[1] 

机构地区:[1]兰州大学第二医院药剂科,兰州730030

出  处:《中国药学杂志》2007年第19期1496-1498,共3页Chinese Pharmaceutical Journal

摘  要:目的建立可同时检测人血浆中替米沙坦、氢氯噻嗪含量的反相高效液相色谱方法。方法色谱条件:色谱柱为ZorbaxsoA Extend-C18(4.6mm×150mm,5μm);流动相为乙腈-四丁基氢氧化铵磷酸盐缓冲液(24:76);流速:1.0mL·min^-1;检测波长:230nm(替米沙坦),272nm(氢氯噻嗪);柱温:20℃。结果本法替米沙坦的线性范围为3l.8~l591.2μg·L^-1,r=0.9994.方法回收率为98.68%,RSD=3.09%,最低检测质量浓度为20μg·L^-1,氢氯噻嗪的线性范围为30~240μg·L^-1,r=0.9991.方法回收率为100.32%,RSD=5.11%,最低检测质量浓度为20μg·L^-1结论本法可以同时测定血浆中替米沙坦和氢氯噻嗪的浓度,具有样品处理简单、方法稳定、有较好的实用性,易于开展药动学及药效学研究。OBJECTIVE To establish a method for simultaneous determination of telmisartan and hydrochlorothiazide in human plasma. METHODS The concentrations of telmisartan and HCTZ were analysed on a Zorbax 80A Extend -C18 (4.6 mm×150 mm,5 μm) column with a mobile phase consisting of tetrabutylammonium hydroxide phosphate buffer solution ( pH 2.26 ) -acetonitrile (76: 24 ) at a flow rate of 1 mL · min^-1, the wavelengthes were 230 and 272 nm, respectively. RESULTS The calibration curve of TMST was linear with the range of 31.8 - 1 591.2 μg · L^-1. The minimum eoncentration was 20 μg · L^-1. The calibration curve of hydrochlo- rothiazide was linear with the range of 30 - 240 μg · L^-1. The minimum concentration was 20 μg · L^-1. CONCLUSION It is a rapid, reproducible method. Clinical application demonstrated that this method was practical and it can be completely used in the study of pharmacokinetics and pharmacodynamics.

关 键 词:高效液相色谱法 替米沙坦 氢氯噻嗪 血浆浓度 

分 类 号:R917[医药卫生—药物分析学]

 

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