亲水型高分子包敷大孔硅胶基质的弱阳离子交换填料用于蛋白质分离  

Protein Separation By Weak Cation Exchange Chromatography With Hydrophilic Polymer-Coated Macroporous Silica Gel

作　　者：吴晓军[1] 杨睦[1] 李兴发 刘国诠[1] 

机构地区：[1]中国科学院化学研究所,北京100080

出　　处：《分析化学》1997年第1期52-55,共4页Chinese Journal of Analytical Chemistry

基　　金：国家科委863资助项目。

摘　　要：报道用亲水型高分子包敷大孔硅胶基质的羧甲基型（ＣＭ）高效弱阳离子交换色谱分离蛋白质的工作。经ＣＭ柱纯化的溶菌酶比活性提高７倍，活性回收率高于９５％，ＣＭ填料对溶菌酶的容量达 ８０ ｍｇ／ｇ。Carboxymethyl weak cation exchanger based on macroporous silica gel coated with hydrophilic polymer was synthesized for the separation of proteins. After purification with the column the specific activity of lysozyme was raised for 7 times with a >95% recovery.

关 键 词：液相色谱 弱阳离子交换剂 包敷硅胶 蛋白质 

分 类 号：O629.73[理学—有机化学] O652.63[理学—化学]