人工组织神经移植物对大鼠陈旧性坐骨神经缺损(60天)的修复作用  

EFFECT OF THE ARTIFICIAL NERVE GRAFT ON NERVE REGENERATION IN RAT SCIATIC NERVE OF TWO MONTHS DEFECT MODEL

作　　者：姚健[1] 施伟[1] 袁颖[1] 林巍巍[1] 陈雪[1] 李奕[1] 王晓冬[1] 

机构地区：[1]南通大学医学院组织学与胚胎学教研室江苏省神经再生重点实验室,南通226001

出　　处：《解剖学报》2007年第5期505-510,共6页Acta Anatomica Sinica

基　　金：江苏省自然科学基金资助项目(BK2002036)

摘　　要：目的观察人工组织神经移植物对陈旧性大鼠坐骨神经缺损的修复作用。方法切除成年SD大鼠部分左侧坐骨神经,饲养60d形成陈旧性坐骨神经缺损后,以人工组织神经移植物修复缺损,同时设自体神经修复和不修复两对照组。修复术后3个月做神经-肌复合电位、腓肠肌湿重及再生神经形态学检测。结果人工组织神经移植物修复组实验侧的运动神经传导速度、腓肠肌湿重、移植物远侧再生神经有髓神经纤维髓鞘厚度等结果与自体神经修复组相似。不修复对照组则未记录到神经-肌复合电位,未观察到再生神经纤维结构,腓肠肌湿重明显小于人工组织神经移植组和自体神经移植组。结论人工组织神经移植物在一定程度上能修复缺损60d的大鼠坐骨神经。Objective The aim of this study was to evaluate the effect of bridging a gap in rat sciatic nerve of 60-day defect with artificial nerve graft. Methods Left sciatic nerves of 20 Sprague-Dawley rats were transected at the mid-thigh level to form nerve defect. After two months, eight rats with artificial nerve graft bridged defect were used as the experiment group. Autologous nerve grafts were used as the positive control in six rats, and another six rats keep defect as the negative control. Three months after the operation, a combination of electrophysiology examination, gastrocnemius muscle wet weight, histological assessment including light microscopy, transmission electron microscopy and immunohistochemistry were utilized to evaluate the nerve repair effects. Results In the artificial nerve graft group, the motor nerve conduction velocity, gastrocnemius muscle wet weight, the average thickness of regeneration myelin sheath and G ratio were similar to those of the autologous nerve graft group. Conclusion Artificial nerve graft can be used for bridging a gap in 60-day nerve defect rat model.

关 键 词：人工组织神经移植物 陈旧性神经缺损 神经再生 神经桥接 大鼠 

分 类 号：R31[医药卫生—基础医学]